Navegando por Palavras-chave "Oral mucositis"

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    Cyclooxygenase-2 and vascular endothelial growth factor expression in 5-fluorouracil-induced oral mucositis in hamsters: evaluation of two low-intensity laser protocols
    (Springer, 2009-11-01) Fontana Lopes, Nilza Nelly [UNIFESP]; Plapler, Helio [UNIFESP]; Chavantes, Maria Cristina; Lalla, Rajesh V.; Yoshimura, Elisabeth Mateus; Seixas Alves, Maria Teresa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Univ Connecticut
    The aim of this study was to investigate the mechanisms whereby low-intensity laser therapy may affect the severity of oral mucositis.A hamster cheek pouch model of oral mucositis was used with all animals receiving intraperitoneal 5-fluorouracil followed by surface irritation. Animals were randomly allocated into three groups and treated with a 35 mW laser, 100 mW laser, or no laser. Clinical severity of mucositis was assessed at four time-points by a blinded examiner. Buccal pouch tissue was harvested from a subgroup of animals in each group at four time-points. This tissue was used for immunohistochemistry for cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), and factor VIII (marker of microvessel density) and the resulting staining was quantified.Peak severity of mucositis was reduced in the 35 mW laser group as compared to the 100 mW laser and control groups. This reduced peak clinical severity of mucositis in the 35 mW laser group was accompanied by a significantly lower level of COX-2 staining. the 100 mW laser did not have an effect on the severity of clinical mucositis, but was associated with a decrease in VEGF levels at the later time-points, as compared to the other groups. There was no clear relationship of VEGF levels or microvessel density to clinical mucositis severity.The tissue response to laser therapy appears to vary by dose. Low-intensity laser therapy appears to reduce the severity of mucositis, at least in part, by reducing COX-2 levels and associated inhibition of the inflammatory response.
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    Efeitos da glicina nas expressões imunohistoquimicas de PDGF, FGF e EGF em mucosite oral induzita em hamster
    (Universidade Federal de São Paulo (UNIFESP), 2017-12-08) Sá, Odara Maria de Sousa [UNIFESP]; Caran, Eliana Maria Monteiro [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Lopes, Nilza Nelly Fontana [UNIFESP]; Maria Teresa de Seixas Alves : http://lattes.cnpq.br/0357765137541523; Nilza Nelly Fontana Lopes : http://lattes.cnpq.br/8672330651019555; http://lattes.cnpq.br/1783139918188371; http://lattes.cnpq.br/3916790331038294; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Oral mucositis is one of the most frequent toxic effects of chemotherapeutic and/or radiotherapeutic treatment, resulting from complex multifaceted biological events involving DNA damage, multiple signaling, and interactions between epithelial, submucosal, and connective tissue. Clinical manifestations such as pain, difficulty in swallowing and communication, and infections have a negative impact on the tolerance to treatment and the quality of life of cancer patients. Preventive measures and curative treatment of mucositis, secondary to chemotherapy and radiotherapy, are still not well established, requiring cooperative studies and new synchronic and effective therapeutic approaches. On the other hand, the nonessential amino acid glycine has anti-inflammatory, immunomodulatory and cytoprotective action, being a potential therapeutic option in mucositis. Therefore, we performed this study with the objective of evaluating the effects of glycine on the expression of collagen and growth factors, platelet (PDGF) and epidermal (EGF), in experimental models of oral mucositis, as apoptotic markers. Methods: The biological material used was the hamster jugal mucosa. The mucositis of which was induced by the protocol proposed by Sonis. A total of 40 hamsters were used, divided into two groups: group I (n= 20)- control; Group II (n= 20) supplemented with 5% intraperitoneal glycine, 0,2 ml (2,0 mg /g) diluted in herpes . On day 7 histopathological sections were performed in order to perform the immune-histochemical method, the evaluation of quantitative and qualitative collagen expression by means of picrossirius under polarized light and the growth factors: EGF and PDGF. Results: It was observed that the group supplemented with glycine higher amounts of collagen expression and predominance type of collagen I when compared to the compared to the control group (p≤0.0002). The glycine group presented lower immunoexpression (p≤0,0001) of the growth factors, EGF and PDGF in relation to the control group. The animals that expressed type I collagen fibers, independent of the experimental group, presented higher amount of collagen and less immunoexpression of the growth factors: EGF and PDGF. Conclusion: The group supplemented with glycine showed a marked healing process of the oral mucosite, demonstrated by the predominance of collagen type I and reduction of growth factors, EGF and PDGF.