Navegando por Palavras-chave "PC-3 cells"

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    Expression and regulation of the estrogen receptors in PC-3 human prostate cancer cells
    (Elsevier Science Inc, 2016) Pisolato, Raisa [UNIFESP]; Lombardi, Ana Paola Giometti [UNIFESP]; Vicente, Carolina Meloni [UNIFESP]; Lucas, Thais Fabiana Gameiro [UNIFESP]; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Porto, Catarina Segreti [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to identify the expression, cellular localization and regulation of classic estrogen receptors ER alpha, and ER beta, ER-alpha 36 isoform and GPER in the androgen-independent prostate cancer cell line PC-3. In addition, we evaluated the relative contribution of these receptors to the activation of the ERK1/2 (extracellular signal-regulated protein kinases) signaling pathway. These four estrogen receptors were detected by Western blot assays and were shown by immunofluorescence assays to localize preferentially in extranuclear regions of PC-3 cells. In addition, treatment with 17 beta-estradiol (E2) (1 mu M) for 24 h led to down-regulation of the classic estrogen receptors, whereas E2 at physiological concentration (0.1 nM) for 24 h tended to increase the levels of ER alpha and ER beta. Furthermore, the ER alpha-selective agonist PPT selectively increased the expression of ER beta and the ER beta-selective agonist DPN increased ER alpha levels. None of these treatments affected expression of the ER-alpha 36 isoform. The unusual cytoplasmic localization of the classic estrogen receptors in these cells differs from the nuclear localization in the majority of estrogen target cells and suggests that rapid signaling pathways may be preferentially activated. In fact, treatment with selective agonists of ER alpha, ER beta and GPER induced ERK1/2 phosphorylation that was blocked by the respective antagonists. On the other hand, activation of ERK1/2 induced by E2 may involve additional mechanisms because it was not blocked by the three antagonists. Taken together, the results indicate that there is a crosstalk between ER alpha and ER beta to regulate the expression of each other, and suggest the involvement of other receptors, such as ER-alpha 36, inthe rapid ERK1/2 activation by E2. The identification of new isoforms of-ERs, regulation of the receptors and signaling pathways is important to develop new therapeutic strategies for the castration-resistant prostate cancer. (C) 2016 Elsevier Inc. All rights reserved.
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    Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles
    (Springer, 2016) Haddad, Paula Silvia [UNIFESP]; Santos, Marconi da Cruz [UNIFESP]; de Guzzi Cassago, Carolina Aparecida; Bernardes, Juliana S.; de Jesus, Marcelo Bispo; Seabra, Amedea Barozzi [UNIFESP]
    Recently, increasing interest is spent on the synthesis of superparamagnetic iron oxide nanoparticles, followed by their characterization and evaluation of cytotoxicity towards tumorigenic cell lines. In this work, magnetite (Fe3O4) nanoparticles were synthesized by the polyol method and coated with polyethylene glycol (PEG) and glutathione (GSH), leading to the formation of PEG-Fe3O4 and GSH-PEG-Fe3O4 nanoparticles. The nanoparticles were characterized by state-of-the-art techniques: dynamic light scattering (DLS), atomic force microscopy (AFM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and superconducting quantum interference device (SQUID) magnetic measurements. PEG-Fe3O4 and GSH-PEG-Fe3O4 nanoparticles have crystallite sizes of 10 and 5 nm, respectively, indicating compression in crystalline lattice upon addition of GSH on the nanoparticle surface. Both nanoparticles presented superparamagnetic behavior at room temperature, and AFM images revealed the regular spherical shape of the nanomaterials and the absence of particle aggregation. The average hydrodynamic sizes of PEG-Fe3O4 and GSH-PEG-Fe3O4 nanoparticles were 69 +/- 37 and 124 nm +/- 75 nm, respectively. The cytotoxicity of both nanoparticles was screened towards human prostatic carcinoma cells (PC-3). The results demonstrated a decrease in PC-3 viability upon treatment with PEG-Fe3O4 or GSH-PEG-Fe3O4 nanoparticles in a concentration-dependent manner. However, the cytotoxicity was not time-dependent. Due to the superparamagnetic behavior of PEG-Fe3O4 or GSHPEG-Fe3O4 nanoparticles, upon the application of an external magnetic field, those nanoparticles can be guided to the target site yielding local toxic effects to tumor cells with minimal side effects to normal tissues, highlighting the promising uses of iron oxide nanoparticles in biomedical applications.