Navegando por Palavras-chave "Primary myelofibrosis"

Agora exibindo 1 - 3 de 3
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Increased angiogenesis in primary myelofibrosis: latent transforming growth factor-β as a possible angiogenic factor
    (Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, 2014-10-01) Ponce, Cesar Cilento; Chauffaille, Maria de Lourdes Lopes Ferrari [UNIFESP]; Ihara, Silvia Saiuli Miki [UNIFESP]; Silva, Maria Regina Regis; Universidade Federal de São Paulo (UNIFESP)
    Objective: The aim of this work was to demonstrate a possible relationship between anti-latency-associated peptide human latent transforming growth factor beta 1 (latent TGF-β1) expression in megakaryocytes and microvascular density in bone marrow biopsies from patients with essential thrombocythemia and primary myelofibrosis. Methods: Microvascular density was evaluated by immunohistochemical analysis and the expression of latent TGF-β1 in samples (100 megakaryocytes per bone marrow sample) from 18 essential thrombocythemia and 38 primary myelofibrosis (19 prefibrotic and 19 fibrotic) patients. Six bone marrow donor biopsies were used as controls. Fibrosis in the bone marrow biopsies was evaluated according to the European Consensus. Results: The average fibrosis grade differed between essential thrombocythemia and primary myelofibrosis groups when compared to the control group. Latent TGF-β1 expression differed significantly between the fibrotic primary myelofibrosis (PMF) group and the control group (p-value < 0.01). A high degree of neo-angiogenesis (demonstrated by analysis of CD34 expression) was detected in patients with myelofibrosis. There were correlations between latent TGF-β1 expression and microvascular density (r = 0.45; p-value < 0.0009) and between degree of microvascular density and fibrosis grade (r = 0.80; p-value < 0.0001). Remarkable differences for neo-angiogenesis were not observed between patients with essential thrombocythemia and controls. Conclusion: Angiogenesis participates in the pathogenesis of primary myelofibrosis, in both the prefibrotic and fibrotic stages, while latent TGF-β is differentially expressed only in the prefibrotic stage.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    MPL immunohistochemical expression as a novel marker for essential thrombocythemia and primary myelofibrosis differential diagnosis
    (Elsevier B.V., 2012-01-01) Ponce, Cesar Cilento [UNIFESP]; Chauffaille, Maria de Lourdes F. [UNIFESP]; Ihara, Silvia Saiuli M. [UNIFESP]; Silva, Maria Regina R. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    To evaluate the grading of fibrosis and immunohistochemical expression of MPL in bone marrow biopsies of ET and PMF. Fibrosis in bone marrow biopsies (BMBs) was evaluated according to the European Consensus for grading of fibrosis, according to reticulin proliferation. Immunohistochemical analysis was performed in samples from 18 ET and 38 PMF patients: 19 were classified as pre-fibrotic and 19 were classified as fibrotic according to the World Health Organization (WHO) criteria, by means of the MPL antibody. Six bone marrow donors' biopsies were used as controls. Average reticulin (p < 0.003) and MPL (p < 0.008) values differed significantly between the ET group and the pre-fibrotic stage PMF group. the MPL immunohistochemical expression may represent a new marker for differential diagnosis between ET and pre-fibrotic stage PMF. (C) 2011 Elsevier B.V. All rights reserved.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    The relationship of the active and latent forms of TGF-beta 1 with marrow fibrosis in essential thrombocythemia and primary myelofibrosis
    (Humana Press Inc, 2012-12-01) Ponce, Cesar Cilento [UNIFESP]; Chauffaille, Maria de Lourdes F. [UNIFESP]; Ihara, Silvia Saiuli M. [UNIFESP]; Silva, Maria Regina R. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to perform an immunohistochemical analysis from 100 megakaryocytes per sample, analyzing positivity and intensity levels of anti-LAP human TGF-beta 1 (or Latent TGF-beta 1) and anti-TGF-beta 1 (or Active TGF-beta 1) antibodies from 18 essential thrombocythemia (ET) and 38 primary myelofibrosis (PMF) patients (being 19 pre-fibrotic and 19 fibrotic). Six bone marrow donor biopsies were used as controls. Fibrosis in bone marrow biopsies (BMB) was evaluated according to the European Consensus. the average fibrosis grade differed between each group (P = 0.001 or P = 0.003). Latent TGF-beta 1 values differed significantly between pre-fibrotic (P = 0.018) and fibrotic (P = 0.031) groups when compared with the control group. the high immunoexpression level of Latent TGF-beta 1 in the megakaryocytes from patients with myelofibrosis, which was not observed in patients with essential thrombocythemia, may be associated with the development of bone marrow fibrosis.