Navegando por Palavras-chave "Protein p53"

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    Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
    (Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED, 2005-03-01) Forones, Nora Manoukian [UNIFESP]; Carvalho, Ana Paula Souza; Giannotti-Filho, Oswaldo; Lourenço, Laércio Gomes [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; IOncology Group Gastroenterology Division; Pathology Department; Universidade Federal de São Paulo (UNIFESP)
    BACKGROUND: Higher proliferation is commonly observed in cancer cells. Apoptosis can be a useful measure of a tumor cell kinetic. Alteration of the balance between proliferation and apoptosis is associated with cancer. AIM: To study proliferation and apoptosis on gastric cancer and in intestinal metaplasia. METHODOLOGY: Twenty-two samples from gastric adenocarcinomas and 22 biopsies from intestinal metaplasia were studied. The apoptotic bodies in hematoxylin-eosin slides and the expression of p53, bcl-2 and Ki67 were determined by immunohistochemistry. RESULTS: The number of the apoptotic cells was higher in cancer. Ki 67LI increased from intestinal metaplasia to gastric cancer. p53 was positive in 68% of the patients with cancer, more frequently in advanced stage and negative in samples of intestinal metaplasia. Although there was no significant difference between the groups, bcl-2 was positive in 45% of gastric cancer tissue and in 68% of metaplasia. In gastric cancer patients bcl-2 was expressed in early gastric cancer more frequently than in advanced stage. CONCLUSION: The positivity of bcl-2 was higher in metaplasia and probably is involved in the progression of carcinogenesis. p53 was negative in metaplasia and positive in more than half of the gastric cancer, mostly in stage IV, suggesting a late event in gastric cancer.
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    Clinical-pathological and p53 protein expression study in dysplasia associated with ulcerative colitis
    (Sociedade Brasileira de Coloproctologia, 2014-09-01) Baldin Júnior, Antônio; Telles, José Ederaldo Queiroz [UNIFESP]; Bonardi, Renato De Araújo; Amarante, Heda Maria Barska Dos Santos; Baldin, Rosimeri Kuhl Svoboda; Universidade Federal do Paraná (UFPR); Universidade Federal do Paraná (UFPR) Hospital de Clínicas Coloproctology Unit; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Paraná (UFPR) Hospital de Clínicas; Universidade Federal do Paraná (UFPR) Department of Clinical Pathology; Universidade Federal do Paraná (UFPR) Department of Surgery; Universidade Federal do Paraná (UFPR) Department of Internal Medicine
    Background: The association between ulcerative colitis and adenocarcinoma determined strategies for patient follow-up and early detection of dysplastic and neoplastic lesions. Aims: To analyze the incidence of dysplasia in patients with ulcerative colitis, comparing clinical data of patients with and without dysplasia and check immunohistochemical expression of p53 protein in dysplasias. Materials and methods: We analyzed biopsy samples and clinical data of 124 patients with ulcerative colitis at Hospital de Clínicas, Federal University of Paraná, Curitiba, Brazil. Results: Dysplasia incidence was low (9.67%) and all cases with low-grade dysplasia. Patients clinical data comparison with and without dysplasia did not show significant statistical differences with regard to the race, age at the start of the disease, age at last biopsy, duration and anatomic extent of ulcerative colitis. Significant difference was found between males and females with predominance of males (58.34%) for dysplasia. Seventeenth biopsy samples of 12 patients with dysplasia, 5 (29.4%) were p53 positive. Conclusions: From these results it is concluded that the incidence of dysplasia was low, higher in males and there was positivity of p53 protein in dysplasia.