Navegando por Palavras-chave "Protein quality control"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Aerobic exercise training rescues protein quality control disruption on white skeletal muscle induced by chronic kidney disease in rats
    (Wiley, 2018) Moraes, Wilson Max Almeida Monteiro de [UNIFESP]; Souza, Pamella Ramona Moraes de; Paixao, Nathalie Alves de; Sousa, Luis Gustavo Oliveira de; Ribeiro, Daniel Araki [UNIFESP]; Marshall, Andrea G.; Prestes, Jonato; Irigoyen, Maria Claudia; Brum, Patricia Chakur; Medeiros, Alessandra [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    We tested whether aerobic exercise training (AET) would modulate the skeletal muscle protein quality control (PQC) in a model of chronic kidney disease (CKD) in rats. Adult Wistar rats were evaluated in four groups: control (CS) or trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise (60 min., five times/wk for 2 months). We evaluated motor performance (tolerance to exercise on the treadmill and rotarod), cross-sectional area (CSA), gene and protein levels related to the unfolded protein response (UPR), protein synthesis/survive and apoptosis signalling, accumulated misfolded proteins, chymotrypsin-like proteasome activity (UPS activity), redox balance and heat-shock protein (HSP) levels in the tibialis anterior. 5/6NxS presented a trend towards to atrophy, with a reduction in motor performance, down-regulation of protein synthesis and up-regulation of apoptosis signalling; increases in UPS activity, misfolded proteins, GRP78, derlin, HSP27 and HSP70 protein levels, ATF4 and GRP78 genes; and increase in oxidative damage compared to CS group. In 5/6NxE, we observed a restoration in exercise tolerance, accumulated misfolded proteins, UPS activity, protein synthesis/apoptosis signalling, derlin, HSPs protein levels as well as increase in ATF4, GRP78 genes and ATF6α protein levels accompanied by a decrease in oxidative damage and increased catalase and glutathione peroxidase activities. The results suggest a disruption of PQC in white muscle fibres of CKD rats previous to the atrophy. AET can rescue this disruption for the UPR, prevent accumulated misfolded proteins and reduce oxidative damage, HSPs protein levels and exercise tolerance.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Efeito do treinamento físico aeróbico na hipertrofia cardíaca de ratos espontaneamente hipertensos: papel do sistema ubiquitina proteassoma
    (Universidade Federal de São Paulo (UNIFESP), 2013-08-28) Andrade, Luiz Henrique Soares de [UNIFESP]; Medeiros, Alessandra [UNIFESP]; http://lattes.cnpq.br/0071198026371230; Universidade Federal de São Paulo (UNIFESP)
    Hypertension is a clinical condition that contributes to the development of cardiac hypertrophy. The activity level of the ubiquitin proteasome system (UPS) is involved in the initiation and progression of cardiac hypertrophy. Moreover, physical training can decrease blood pressure, reduce or reverse the pathological cardiac hypertrophy and this response may, at least in part, occur through changes in the activity of the SUP in the heart. The purpose of this study was to investigate the effect of aerobic exercise training in cardiac hypertrophy and activity of UPS in spontaneously hypertensive rats (SHR). For this, we used Wistar and SHR rats (8 weeks old) randomly divided into sedentary and trained, and the exercise training protocol was 5x/week in treadmill, low intensity, 60 min, for 13 weeks. Before, during and after the experimental period exercise tolerance test was performed. The recording indirect systolic blood pressure (SBP), heart rate (HR) and body weight were evaluated once a week. The proteasome activity in the catalytic site of chymotrypsin was measured by fluorimetric assay. The tr aining significantly increased exercise tolerance in hypertensive animals, decreased SBP and HR at rest after the period, and elevated diastolic function. In addition, it restored the proteasome activity on the control groups levels. Data from this study demonstrated the important non-pharmacological therapeutic effect of exercise on ubiquitin proteasome system in hypertension.