Navegando por Palavras-chave "Pulsed-field gel electrophoresis"

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    Emergence of nosocomial Mycobacterium massiliense infection in Goias, Brazil
    (Elsevier B.V., 2008-11-01) Cardoso, Alessandra Marques; Sousa, Eduardo Martins de; Viana-Niero, Cristina [UNIFESP]; Bortoli, Fernando Bontim de; Pereira das Neves, Zilah Candida; Leao, Sylvia Cardoso [UNIFESP]; Junqueira-Kipnis, Ana Paula; Kipnis, Andre; Universidade Federal de Goiás (UFG); Universidade Federal de São Paulo (UNIFESP); Secretaria Municipal Saude Goiania
    A cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiania, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiania apart from this period. the 18 isolates were identified as Mycobacterium massiliense by PCR-restriction digestion of the hsp65 gene, pulsed-field gel electrophoresis (PFGE) comparisons, and rpoB partial gene sequencing. All isolates were typed as a single clone, indicating that they have the same origin, which suggests a common source of infection for all patients. (C) 2008 Elsevier Masson SAS. All rights reserved.