Navegando por Palavras-chave "Resposta a proteína mal enovelada"

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    Efeitos do treinamento físico aeróbio sobre a resposta à proteína mal enovelada na musculatura esquelética de ratos submetidos à uremia experimental
    (Universidade Federal de São Paulo (UNIFESP), 2016-07-28) Moraes, Wilson Max Almeida Monteiro de [UNIFESP]; Medeiros, Alessandra [UNIFESP]; http://lattes.cnpq.br/0071198026371230; Universidade Federal de São Paulo (UNIFESP)
    The present study tested whether aerobic exercise training (AET) would modulate the unfolded protein response (UPR) in a model of Chronic Kidney Disease (CKD) in rats. Adult Wistar rats were evaluated in 4 groups: control (CS), control trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise for 60 min, 5 times/wk for 2 months. We evaluated motor performance (tolerance to exercise in treadmill and rotarod), cross sectional area (AST), gene and protein levels related to UPR, protein synthesis/survive and apoptosis signaling, accumulated misfolded proteins, chymotripsin-like proteasome activity (UPS activity), redox balance and HSPs protein levels in tibialis anterior. Despite the AST were not different between groups, the 5/6NxS presented a reduction in motor performance followed by down regulation in protein synthesis and up regulation of apoptosis signaling, increased UPS activity, misfolded proteins, GRP78, derlin, HSP27 and HSP70 protein levels and ATF4 and GRP78 genes, increased in oxidative damage compared to CS group. In 5/6NxE, we observed restoration in exercise tolerance, accumulated misfolded proteins, UPS activity, protein synthesis and apoptosis signaling, derlin and HSPs protein levels as well as increased in ATF4 and GRP78 genes and GRP78,ATF6? protein levels accompanied by an decrease in oxidative damage and increased catalase and glutathione-peroxidase activities. These results suggest that an UPR is activated in white muscle fibers of CKD rats, independently of atrophy and that AET amplified this response, but prevented accumulated misfolded proteins, promoting reduction in oxidative damage, HSPs protein levels and exercise tolerance.