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- ItemAcesso aberto (Open Access)Análise proteômica comparativa de isolados de p. brasiliensis e sua relação com a virulência fúngica(Universidade Federal de São Paulo (UNIFESP), 2018-06-07) Amaral, Cristiane Candida do [UNIFESP]; Camargo, Zoilo Pires de [UNIFESP]; http://lattes.cnpq.br/0632912481397728; http://lattes.cnpq.br/9021981707387327Paracoccidioidomycosis (PCM) is a systemic mycosis commonly found in Latin America that is caused by two distinct species of Paracoccidioides genus, Paracoccidioides brasiliensis complex (S1, PS2, PS3 e PS4) and Paracoccidioides lutzii. Its pathobiology has been recently explored by different approaches to clarify the mechanisms of host-pathogen interactions underpinning PCM. The diversity of clinical forms of this disease has been attributed to both host- and fungus-related factors. For better understanding of the molecular underpinnings of host-fungus interactions, we evaluated in vivo virulence of nine Paracoccidoides brasiliensis isolates and correlated it to protein expression profiles obtained by two-dimensional gel electrophoresis. Based on the recovery of viable fungi from mouse organs, the isolates were classified as those having low, moderate, or high virulence. Highly virulent isolates overexpressed proteins related to adhesion process and stress response, probably indicating important roles of those fungal proteins in regulating the colonization capacity, survival, and ability to escape host immune system reaction. Moreover, highly virulent isolates exhibited enhanced expression of glycolytic pathway enzymes concomitantly with repressed expression of succinyl-CoA ligase beta chain, a protein related to the tricarboxylic acid cycle. Our findings may point to the mechanisms used by highly virulent P. brasiliensis isolates to withstand host immune reactions and to adapt to transient iron availability as strategies to survive and overcome stress conditions inside the host.