Navegando por Palavras-chave "Restrição de crescimento intrauterino"
Agora exibindo 1 - 2 de 2
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)O efeito da administração pós-natal de topiramato na programação metabólica de camundongos submetidos a um protocolo de restrição de crescimento intrauterino(Universidade Federal de São Paulo (UNIFESP), 2018-02-22) Oliveira, Allan Chiaratti de [UNIFESP]; D'Almeida, Vania [UNIFESP]; http://lattes.cnpq.br/7220411418339421; http://lattes.cnpq.br/9749043459728526; Universidade Federal de São Paulo (UNIFESP)Obesity is a public health issue with epidemic proportions. Intrauterine growth restriction negatively impacts immediate neonatal outcome and potentially increases the risk of chronic non-transmissible diseases posteriorly in adult life. Animal models have been used to understand the mechanisms underlining these long-term consequences. The search for strategies that could attenuate the impact of intrauterine growth restriction in latter life health is encouraging. Topiramate is an anticonvulsive drug with metabolic effects akin to those involved in metabolic programming. Intrauterine growth restriction was elicited by protein restriction prior and during gestation in female mice. Pups were treated either with topiramate or the vehicle during the suckling period and were challenged with hyperlipidic hypercaloric diet in adulthood. Protein restriction during gestation was able to reduce 36% the birth weight in the mice offspring, and despite slower catch up growth, equivalent weights were observed in restricted and control, male and female mice, at the time the nutritional challenge was initiated. Male and female offspring presented specific physiologic responses to the hyperlipidic diet. Male control mice presented reduced weight gain and reduced adiposity after the nutritional challenge, with no differences in total cholesterol, triglycerides, glucose, leptin or insulin plasma levels between the groups. In the other hand, female animals presented differential responses in leptin and insulin plasma levels, with no differences in other biochemical parameters and body weight or composition. Expression of genes related to leptin and adiponectin cross-talk between adipose tissue, liver, muscle and hypothalamus, as well as genes involved in the control of energetic metabolism in the hypothalamus was not changed in male mice after hyperlipidic diet. Female restricted mice treated with the vehicle presented reduced Ampk messenger RNA (mRNA) expression in muscle, what was attenuated in the restricted female mice treated with topiramate. Restricted female mice treated with topiramate also presented reduced Adiponectin receptor 2 and increased Pomc mRNA expression in the hypothalamus, along with increased Mch and decreased Pre-pro-orexin mRNA expression. Neonatal topiramate administration may have attenuated the programming effect of intrauterine growth restriction in female mice, leading to a most favorable organization of the hypothalamic circuitry involved in energetic metabolism control and increased leptin and insulin sensibility.
- ItemAcesso aberto (Open Access)Efeito da restrição proteica gestacional sobre parâmetros morfométricos e metabólicos e sobre o comportamento alimentar na prole de camundongos(Universidade Federal de São Paulo (UNIFESP), 2016-07-31) Silva, Luana Cristina de Almeida [UNIFESP]; D'Almeida, Vania [UNIFESP]; http://lattes.cnpq.br/7220411418339421; http://lattes.cnpq.br/0784370244797321; Universidade Federal de São Paulo (UNIFESP)Objective: The objective of this study was to analyze the metabolic and behavioral effects of fetal growth restriction (IUGR) caused by the deficiency of protein during intrauterine life before and after exposure to high-fat diet. Method: After protein restriction (5g protein / 100 g diet, HP diet) in the maternal diet offspring, it was compared with offspring of mothers receiving normal protein diet (20 g protein / 100 g diet, NP diet). After birth, all offspring were suckled by progenitors fed NP diet and, there was adoption of all litters by progenitors fed NP diet. In postnatal day (PND) 28 a total of 51 offspring were distributed into two groups according to the maternal diet, namely: control group (CT, HP maternal diet) and restriction group (RT, NP maternal diet). The offspring were accompanied for 2 months receiving NP diet and, after this period all of them were fed with a high-fat diet with high concentration of saturated fat (31g saturated fat, 100g diet, HL) for about 5 weeks. The body weights of the offspring were accompanied weekly from birth to the 1st month of life and, monthly from 1st month until euthanasia. The naso-anal length was measured in PND30 and PND60. Blood samples were collected in PND30, PND60 and PND90 to assess blood concentrations of glucose, total cholesterol and triglycerides in whole blood and plasma concentrations of insulin and leptin. The animals were subjected to behavioral tests: Novelty suppressed-feeding test and open field. After behavioral assessments, the animals were euthanized by decapitation and had blood, fat, liver, kidney and muscle collected. Data is presented as mean ± standard deviation. Results and conclusions: IUGR decreased the birth weight of offspring and this effect can be observed at other times throughout the study; in males, this restriction reduced the naso-anal growth and altered muscle mass and the proportion of fat after high fat diet consumption. After spending the period stipulated feeding only at HL diet, restricted animals showed elevated plasma insulin. Males and females RT group showed increased locomotor activity, restricted females also had fewer episodes of self-cleaning during behavioral 95 testing and showed a higher consumption of HL diet over time in relation to the females CT group. With these data, we can conclude that the deficiency in maternal nutrition delayed intrauterine growth, and this effect can interfere in the post-natal development, affecting morphometric parameters before and after consumption of a HL diet. After passing through metabolic challenge exposure (HL diet), restricted animals showed elevated plasma insulin, demonstrating that IUGR may influence metabolic signaling involved in energy homeostasis promoted by a nutritional imbalance.