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    Aplicações de nanopartículas de ouro em doenças de crescimento não controlável
    (Universidade Federal de São Paulo (UNIFESP), 2020-06-26) Goncalves, Karina De Oliveira [UNIFESP]; Courrol, Lilia Coronato [UNIFESP]; Universidade Federal de São Paulo
    Cancer and atherosclerosis are among the leading causes of death in Brazil and worldwide. Despite all the resources available for the treatment of these diseases, a variety of problems are observed, both about the effectiveness of the drugs and the range of effects that can be induced by them. In this context, metallic nanoparticles appear like an alternative, for the diagnosis and therapy of these diseases. Thus, visa work produces metallic nanoparticles (silver and gold) and heterodimer nanoparticles (silver/iron, silver/gold and gold/iron) and tests them as possible drug transport agents and as sensitizers for therapies against cancer and atherosclerosis. In the first step, describing the process of demonstrating 7 nanoparticles, using the photoreduction method, none of which nanoparticles are functional with aminolevulinic acid (5-ALA) or its methyl ester (MALA). Characterization techniques employed were: UV/Vis Spectroscopy, Zeta Potential, Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and X-Ray Diffraction (XRD). The potential for single oxygen generation by nanoparticles was also evaluated, using a DPBF (1,3-diphenyl isobenzofuran) probe or reagent, which combined a nanoparticle solution, was exposed to the LED at 590 nm or Ultrasound with pulses of 1 MHZ and 1W/cm2. DPBF decay measurements were not performed on the UV/Vis spectrophotometer. In the second stage, cytotoxicity tests were performed with murine melanoma cell lines (B16F-10), human monocytes (THP-1 - differentiated into macrophages) and breast tumor (MCF-7) to evaluate how they were less tested in nanoparticles. Cytotoxic effects after photodynamic therapies (PDT) with 590 nm LED (~ 100 mW) and sonodynamics (SDT) with ultrasound with pulses of 1 MHZ and 1W/cm2 were also analyzed, with a price assessment test 5-ALA/MALA by nanoparticles and consequent increase in the production of single oxygen. The results obtained indicate that nanoparticles can deliver 5-ALA / MALA to cells. Among the therapies, an SDT produces better results in conjunction with nanoparticles, applies the potential application of produced nanoparticles, in diseases of uncontrollable growth.