Navegando por Palavras-chave "Species Complex"

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    Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
    (Wiley, 2016) Figueiredo, Thais Pandini; de Lucas, Rosymar Coutinho; Cazzaniga, Rodrigo Anselmo; Franca, Carolina Nunes [UNIFESP]; Segato, Fernando; Taglialegna, Rafael; Maffei, Cláudia Maria Leite
    Cryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compared
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    Molecular identification of Histoplasma capsulatum using rolling circle amplification
    (Wiley-Blackwell, 2016) Furuie, Jason L.; Sun, Jiufeng; do Nascimento, Mariana M. F.; Gomes, Renata R.; Waculicz-Andrade, Caroline E.; Sessegolo, Gisele C.; Rodrigues, Anderson M. [UNIFESP]; Galvao-Dias, Maria A.; de Camargo, Zoilo P. [UNIFESP]; Queiroz-Telles, Flavio; Najafzadeh, Mohammad J.; de Hoog, Sybren G.; Vicente, Vania A.
    Histoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, Histoplasma capsulatum, which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of H. capsulatum infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (RCA) detection method which targets ITS (Internal Transcribed Spacer) rDNA of H. capsulatum enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (HcPL2) allowed specific amplification of H. capsulatumDNA while no cross-reactivity was observed with fungi used as negative controls. This method proved to be effective for H. capsulatum specific identification and demonstrated to be faster than the traditional method of microbiological identification.
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    Molecular identification of Histoplasma capsulatum using rolling circle amplification
    (Wiley-Blackwell, 2016) Furuie, Jason L.; Sun, Jiufeng; do Nascimento, Mariana M. F.; Gomes, Renata Rodrigues; Waculicz-Andrade, Caroline E.; Sessegolo, Gisele C.; Rodrigues, Anderson Messias [UNIFESP]; Galvao-Dias, Maria Adelaide; Camargo, Zoilo Pires de [UNIFESP]; Queiroz-Telles, Flavio; Najafzadeh, Mohammad J.; de Hoog, Sybren G.; Vicente, Vania Aparecida; Universidade Federal de São Paulo (UNIFESP)
    Histoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, Histoplasma capsulatum, which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of H. capsulatum infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (RCA) detection method which targets ITS (Internal Transcribed Spacer) rDNA of H. capsulatum enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (HcPL2) allowed specific amplification of H. capsulatumDNA while no cross-reactivity was observed with fungi used as negative controls. This method proved to be effective for H. capsulatum specific identification and demonstrated to be faster than the traditional method of microbiological identification.