Navegando por Palavras-chave "Spinal cord regeneration"
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- ItemSomente MetadadadosTerapia a laser de baixa intensidade na neuroplasticidade e recuperação funcional da lesão medular(Universidade Federal de São Paulo (UNIFESP), 2016) Campo, Paula Fernanda Gallani Martin Del [UNIFESP]; Aguiar, Odair [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: The spinal cord injury (SCI) consists of any damages to the structures in the medullary canal. This injury can be traumatic or non-traumatic, the latter being the most common. The central nervous system (CNS) has a low ability to get repaired, requiring the use of therapeutic approaches in order to favor neuroplasticity. In this context, low-level laser therapy (LLLT) has been proposed as a promising treatment for repairing central and peripheral nervous system injuries. Aim: to investigate the effects of LLLT, after traumatic injuries, on the regeneration and neuroplasticity of the spinal cord, as well as on the sensorial and motor functional recovery. Materials and methods: female wistar rats (n= 33), aging 10 weeks, were submitted to spinal cord injury contusion model (CEUA N: 9329290514). The animals were divided into two groups: control (C; n= 19) and laser (L; n= 14), and they were euthanized after 4 and 8 weeks. The L group was treated, for 15 consecutive days, with continuous emission of GaAlAs laser which was adjusted with the following parameters: λ = 808 nm, power of 100 mW, spot area = 0.028 cm²; t = 4'42 ''; dosage of 1000 J / cm²; E = 28 J; irradiance = 3.57 mW / cm². At days 1, 14, 28 and 56 after the surgery, the motor function (using BBB score and computed kinematics), the thermal sensitivity (using ice) and the tactile sensitivity (using Von Frey® monofilaments) were evaluated. Additionally, histological and immunohistochemical analysis were performed to investigate the lesion volume and the expression of GFAP respectively. Results: The main findings of this study demonstrate, based on the BBB score, that LLLT was able to improve the motor performance in the group L compared to group C after 56 days. Furthermore, LLLT was capable to decrease the immunoexpression of of GFAP marker and the lesion volume in the injury site after 56 days. Conclusion: Based on the results, it is suggested that the effect of LLLT in the lesion (i) assisted in the motor recovery; (ii) reduced the lesion volume and (iii) decreased the GFAP immunostaining. This study indicates that the LLLT is a promising therapeutic approach for the SCI treatment, justifying the need of further investigations utilizing other experimental models and other specific markers.
- ItemAcesso aberto (Open Access)Traumatic spinal cord injury: current concepts and treatment update(Assoc Arquivos Neuro- Psiquiatria, 2017) Rouanet, Carolina [UNIFESP]; Reges, Danyelle [UNIFESP]; Rocha, Eva Carolina Andrade [UNIFESP]; Gagliardi, Vivian Dias Baptista [UNIFESP]; Silva, Gisele Sampaio [UNIFESP]Spinal cord injury (SCI) affects 1.3 million North Americans, with more than half occurring after trauma. In Brazil, few studies have evaluated the epidemiology of SCI with an estimated incidence of 16 to 26 per million per year. The final extent of the spinal cord damage results from primary and secondary mechanisms that start at the moment of the injury and go on for days, and even weeks, after the event. There is convincing evidence that hypotension contributes to secondary injury after acute SCI. Surgical decompression aims at relieving mechanical pressure on the microvascular circulation, therefore reducing hypoxia and ischemia. The role of methylprednisolone as a therapeutic option is still a matter of debate, however most guidelines do not recommend its regular use. Neuroprotective therapies aiming to reduce further injury have been studied and many others are underway. Neuroregenerative therapies are being extensively investigated, with cell based therapy being very promising.