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    Biocompatibilidade do colageno marinho (espongina) extraida de esponjas marinhas para utilizacao na area de engenharia tecidual
    (Universidade Federal de São Paulo (UNIFESP), 2020-03-03) Santana, Alan De Franca [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Universidade Federal de São Paulo
    Bone tissue is a specialized system and able to regenerate, but in some situations, which can be a fracture due to over load, accidents, pathology, aging. The context of fracture incidence increases significantly, representing a serious public health problem. Estimating about 6.2 million annual fractures in the United States, and this 10% progress to cases of non-consolidation and pseudarthrosis. Generating an expense of approximately 25 thousand cases in cases of pseudarthrosis. Other therapeutic sources have been investigated in order to assist the evaluation process when consequences arise in the consolidation process. Thus biomaterials are gaining prominence as an alternative in the treatment of fractures, being that of collagen origin one of the most used in the area of tissue engineering for its irritability index, good biodegradation and biocompatibility. However, most of this material is of bovine or porcine origin and may be at risk of transmission and possibility of transmission of viruses and bacterial infections. In addition, it may have been paid for an extraction and manufacture of these sources, reflecting in high prices. For the research non marine environment emerge as an alternative to prospecting new sources of raw material to biomaterials. Among the organisms a sponge, the keyword is a structural component, is a piece called inorganic called Basilica, and a part is called sponge, is an analog of type XIII. Aplyina fulva fluidin, in vitro and in vivo of spp. A characterization made by SEM / EDX, FTIR, pH Assay and Mass. Collagen was used for comparison in pH and mass analysis. The SEM / EDX results were displayed as a PES, showed irregular presence numbers of Al (Aluminum), C (Carbon), O (Oxygen) and S (Sulfur), Ca (Calcium), Cl (Chlorine), Fe ( Iron), Ti (Magnesium), Na (Sodium), P (Phosphorus) and Si (Silicon). The FTIR results were possible observing the peaks referring to the O-H, N-H and C-O groups. In pH analysis it is observed that on day 3 that ESP acidifies the incubation medium, and from day 7 onwards an alkalinization occurs, while COL acidifies the medium on day 3 and alkalizes the new walk near physiological pH again from day one. 14. Results were negative for both groups and are observed at a mass loss from incubation period to day 14. Results of histological analysis compared to the amount of days after formation of newly formed bone tissue and You can have a wide variety of components to store material waste. In the in vivo assays it was observed that in the different concentrations of ESP used, did not inhibit the cell proliferation of the used strains, showing no cytotoxic effects. Therefore, for future stages, genotocixity assays will be performed through the Comet and Micronucleus tests to evaluate DNA damage.