Navegando por Palavras-chave "Transcription factors"
Agora exibindo 1 - 5 de 5
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)Diabetes Mellitus do Tipo MODY(Sociedade Brasileira de Endocrinologia e Metabologia, 2002-04-01) Oliveira, Carolina Soares Viana de [UNIFESP]; Furuzawa, Gilberto K. [UNIFESP]; Reis, André Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)It is estimated that close to 5% of the individuals classified as having type 2 diabetes mellitus (DM) and about 10% of those considered type 1 DM (previously categorized as juvenile type) are actual carriers of a MODY mutation. In this form of DM, there is evident co-segregation of some mutations and the advent of hyperglycemia, this fact having been reproduced by the study of several families of different populations. Its main characteristic is being one of the few causes of DM in which the transmission of the genetic susceptibility is due to an autossomical dominant inheritance, making part of the group classified as monogenic DM, where the other members are very rare. Mutations occurring in MODY genes, even in the heterozygous form, lead to a profound phenotypic impact (high penetrance), in that 95% of the individual carriers of a MODY mutation will be diabetic or will have altered glicemic metabolism before the age of 55 years. In this paper we approach this form of DM, emphasizing its most relevant clinical and genetic characteristics. The systematic search for MODY mutations is beginning to take place regularly in many countries, and there is a tendency to add this diagnostic tool to the routine exams in the practice of diabetology.
- ItemAcesso aberto (Open Access)O fator de transcrição NF-kapaB nos mecanismos moleculares de ação de psicofármacos(Associação Brasileira de Psiquiatria - ABP, 2000-03-01) Glezer, Isaias [UNIFESP]; Marcourakis, Tania; Avellar, Maria Christina Werneck [UNIFESP]; Gorenstein, Clarice; Scavone, Cristoforo; Universidade de São Paulo (USP); Centro de Investigações Médicas em Neurologia; Universidade Federal de São Paulo (UNIFESP)During the last years many efforts have been made in order to elucidate the mechanisms involved in the gene transcription regulation. Special attention has been given to some molecules involved in these regulatory processes, as the transcription factors. Understanding the role of these factors in several neural functions will allow a better knowledge of the disorders related to the central nervous system (CNS) disorders, and will also help identify new pathways for therapeutic access. The transcription factor NF-kappaB is remarkable for its wide range of actions and also due to the many different proteins involved in its activation. There is evidence this factor works in the plasticity, development and neurodegeneration, and takes part of essential and specific functions of neurons and glial cells. Brain-specific activators of NF-kappaB include glutamate (via both NMDA and AMPA/KA receptors) and neurotrophins. This article reviews the complex biochemical regulation of NF-B activation, emphasizing the potential of the contributions this transcription factor could make to psychopharmacology field. Therefore, changes in NF-kappaB activity might provide insight in the development of new psychoactive drugs.
- ItemAcesso aberto (Open Access)High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model(Natl Inst Science Communication-Niscair, 2017) do Carmo, Luciana Simao; de Oliveira, Dalila Cunha; Cortez, Mayara; Nogueira-Pedro, Amanda [UNIFESP]; Paredes-Gamero, Edgar Julian [UNIFESP]; Borelli, Primavera; Rogero, Marcelo Macedo; Fock, Ricardo AmbrosioExcessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies.
- ItemAcesso aberto (Open Access)Papel de oct4, sox2 e caspase-3 na quiescência dos gonócitos de rato(Universidade Federal de São Paulo (UNIFESP), 2014-10-29) Arantes, Anelise Diniz [UNIFESP]; Teixeira, Taiza Stumpp Teixeira [UNIFESP]; Oliva, Samara Urban de [UNIFESP]; http://lattes.cnpq.br/5886968082690915; http://lattes.cnpq.br/1024329256080770; http://lattes.cnpq.br/0559069185165585; Universidade Federal de São Paulo (UNIFESP)Introduction: The germ cells emerge from the epiblast and migrate to the gonads, where they become gonocytes. The gonocytes are the precursors of the spermatogonial stem cells, but little is known about their differentiation. It is seems that the rigid control of gonocyte proliferation, quiescence and pluripotent marker expression is crucial for germ cell development. Objective: Identify the pre-natal period of male germ cell quiescence in the rat and whether caspase-3 (Casp3) is involved in the process. Methods: Rat embryonic gonads were collected at 15, 17 and 19 days post-coitum (dpc). The expression of pluripotency (SOX2, OCT4) and proliferation (Ki67, phosphorylated Retinoblastoma 1 (pRb1)) markers as well as of cleaved Casp3 was analysed. To address Casp3 role in rat gonocyte quiescence, testes from 19dpc embryos were incubated with Casp3 inhibitor and submitted to the immunolabelling of Ki67, PCNA, Casp3 and NANOG. Results: The number of Ki67 and pRB1-labelled gonocytes reduced from 15dpc to 17dpc reaching zero at 19dpc, indicating that quiescence starts around 15dpc and that they are quiescent at 19dpc. OCT4 labelling followed the same detection pattern, whereas SOX2 was present only at 15dpc. These data suggest that the establishment of the quiescence period involves the downregulation of these pluripotent markers. Casp3 labelling was opposite to OCT4, pRB1 and Ki67 labelling, suggesting that it has a role in rat gonocyte quiescence. Casp3 labelling was maintained after the incubation with Casp3 inhibitor, what was expected since the inhibitor does not induce Casp3 degradation. Ki67 was not detected in the gonads incubated with Casp3 inhibitor, suggesting that Caps3 inhibition does not reactivate the cell cycle. NANOG was detected in the gonocyte cytoplasm at 19dpc and its labelling was identical to that of Casp3, suggesting that it has a role in the control of germ cell cycle in the embryo and may interact with Casp3. PCNA-positive and negative gonocytes were observed in 19dpc gonads before and after culture. However, the number of PCNA-negative gonocytes increased in the gonads incubated with Casp3-inhibitor, suggesting that Casp3 is a positive regulator of PCNA. Conclusion: These results suggest that OCT4 and SOX2 downregulation as well as Casp3 and NANOG expression are involved in rat gonocyte quiescence and that PCNA depend on Casp3 activity in these cells.
- ItemSomente MetadadadosPluripotent stem cell transcription factors during human odontogenesis(Springer, 2013-09-01) Cunha, Juliana Malta da [UNIFESP]; Costa-Neves, Adriana da; Kerkis, Irina; Pereira da Silva, Marcelo Cavenaghi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Inst ButantanStem cells are capable of generating various cell lines and can be obtained from adult or embryonic tissues for clinical therapies. Stem cells from deciduous dental pulp are among those that are easily obtainable from adult tissues and have been widely studied because of their ability to differentiate into a variety of cell lines in the presence of various chemical mediators. We have analyze the expression of several proteins related to the differentiation and proliferative potential of cell populations that compose the tooth germ of human fetuses. We evaluate 20 human fetuses of both genders. After being paraffin-embedded, cap and bell stages of tooth germ development were subjected to immunohistochemistry for the following markers: Oct-4, Nanog, Stat-3 and Sox-2. the studied antibodies showed nuclear or cytoplasmic immunnostaining within various anatomical structures and with various degrees of expression, indicating the action of these proteins during tooth development. We conclude that the interrelationship between these transcription factors is complex and associated with self-renewal and cell differentiation. Our results suggest that the expression of Oct-4, Nanog, Sox-2 and Stat-3 are related to differentiation in ameloblasts and odontoblasts.