Navegando por Palavras-chave "Trypanossoma Cruzi"

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    Caracterização funcional da histona H2B variante em diferentes formas de vida de Trypanosoma cruzi
    (Universidade Federal de São Paulo (UNIFESP), 2019-01-30) Roson, Juliana Nunes [UNIFESP]; Cunha, Julia Pinheiro Chagas da [UNIFESP]; http://lattes.cnpq.br/5439604707221039; http://lattes.cnpq.br/3535401560274603; Universidade Federal de São Paulo (UNIFESP)
    The differentiation of Trypanosoma cruzi is followed by changes in parasite morphology and a complete reorganization of the nucleus and chromatin structure. Studies indicate that the deposition of histone variants changes chromatin structure, possibly affecting gene regulation. Recently, in our group it was verified that histone H2BV is enriched in the chromatin of trypomastigote forms of T. cruzi. In this dissertation, we sought to understand the role of histone H2BV in the life forms of T. cruzi. For this, heminoucautes parasites were generated for H2BV (H2BV-HtzKO) and the interaction partners of this protein were evaluated. In general, H2BV-HtzKO clones exhibit higher rates of proliferation and differentiation in metacyclics over wild-type parasites. In addition, they have a higher number of cells in the S phase of the cell cycle. By quantitative proteomics, it has been confirmed that H2BV expression is decreased in chromatin and H2BV-HtzKO parasites and, interestingly, we have found that the other histone variants (H2A.Z and H3V) are also less abundant. In addition, the abundance of other histones (namely, H4, H2A and H1) is increased in the H2BVHtzKO parasites suggesting a compensatory mechanism in histone deposition at the chromatin. Pulldown assays were performed using the H2BV and canonical H2B recombinants (as a control) to identify their specific interaction partners in the epimastigote or trypomastigote forms. Many interesting, proteins have been identified, but the bromodomain-2 factor (BDF2) is exclusively found in trypomastigote extracts. It is known that BDF2 recognizes histone acetylation and, according to the same, we find that H2BV itself is acetylated at residue K97. The study of proteins with epigenetic function as highlighted in this dissertation are important for a better understanding of the biology of trypanosomes since they participate in pathways essential for the growth / differentiation of the parasite and thus may also be excellent therapeutic prototypes for trypanosomiasis.
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    Resposta das células T CD8+ em modelos de obesidade infectados pelo Trypanosoma Cruzi
    (Universidade Federal de São Paulo (UNIFESP), 2018-03-19) Correa, Priscila Gontijo [UNIFESP]; Vasconcelos, Jose Ronnie Carvalho de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Chagas Disease Is An Infection Transmitted By The Intracellular Protozoan Trypanosoma Cruzi. The Specific Immune Response Mediated By Cd8 + T Cells Are Crucial To Control Infection, And Changes In Response Of These Cells Increases The Risk Of Morbidity And Mortality By The Disease. The Excess Of Adipocytes, Characteristic Of Obesity, Potentiates The Inflammatory Process From The Infection, Which Could Interfere In The Response Of The Specific Cd8 + T Cells. Objective: Assess The Response Of The Specific Cd8+ T Cells Generated In The Infection Of The Trypanossoma Cruzi, In Different Models Of Obesity. Material And Methods: Two Murine Models Of Obesity Were Studied, One Genetically Linked And Another Induced By High Fat Diet (Hfd) Consumption. For Genetic Obesity, Ob/Ob Fat And Ob/Ob Lean Mice Were Used Animals. For Hfd-Induced Obesity Model, C57bl/6 Mice Were Fed A High Fat Diet, Containing 39% Of Fat. The Two Models Were Infected With T. Cruzi, And Blood Parasitemia, Survival And Immune Response