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- ItemAcesso aberto (Open Access)Consumo de frutas e hortaliças por adultos em Ribeirão Preto, SP(Faculdade de Saúde Pública da Universidade de São Paulo, 2010-08-01) Mondini, Lenise [UNIFESP]; Moraes, Suzana Alves de; Freitas, Isabel Cristina Martins de; Gimeno, Suely Godoy Agostinho [UNIFESP]; Secretaria de Agricultura e Abastecimento do Estado de São Paulo Instituto de Economia Agrícola; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)OBJECTIVE: To assess fruit and vegetable intake by adults and identify sociodemographic and life style variables associated with this intake. METHODS: A population-based cross-sectional study was performed in the urban area of the city of Ribeirão Preto, Southeastern Brazil, in 2006. Sample was selected using three-stage cluster sampling, with census tracts as primary units. Sample was comprised of 930 participants aged 30 years and more and the design effect was considered in data analysis. Fruit and vegetable intake was based on a semi-quantitative food frequency questionnaire, using mean scores of frequency of fruit and vegetable intake, daily intake and minimum intake of such foods as indicators. The independent variables analyzed were as follows: age group, marital status, level of education, per capita household income, nutritional status, smoking and physical activity. Mean scores of frequency of intake were estimated by point and 95% confidence intervals. Prevalence ratios were also estimated by point and 95% confidence intervals to analyze association, using Poisson regression. Linear trend tests were applied, adopting a 5% confidence level. RESULTS: Only 24% of men and 38% of women met the minimum recommendation for fruit and vegetable intake; there was a positive association with age and per capita income. Women with a higher level of education and men who lived with a female partner consumed more fruits and vegetables than others. Physical activity, smoking and nutritional status were not associated with the minimum recommended fruit and vegetable intake. CONCLUSIONS: Socioeconomic factors have an important influence on fruit and vegetable intake and, as these are subject to intervention, they can contribute to the adoption of healthy eating habits.
- ItemSomente MetadadadosDietary predictors of serum total carotene in low-income women living in São Paulo, south-east Brazil(Cambridge Univ Press, 2009-11-01) Tomita, Luciana Y.; Almeida, Lana C.; Roteli-Martins, Cecilia; D'Almeida, Vania [UNIFESP]; Cardoso, Marly A.; BRINCA Study Team; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Hosp Leonor Mendes BarrosObjective Dietary intake and nutritional status of antioxidant vitamins have been reported to protect against some cancers the objective of the present study was to assess the correlations between serum levels of carotenoids (including beta-, alpha- and gamma-carotene), lycopene, retinol, alpha- and gamma-tocopherols, and dietary intakes estimated by an FFQ, among low-income women in the Brazilian Investigation into Nutrition and Cervical Cancer Prevention (BRINCA) study.Design Cross-sectional study of data for 918 women aged 21-65 years participating in the BRINCA study in São Paulo city. Multiple linear regression models were used with serum nutrient levels as the dependent variable and dietary intake levels as the independent variable, adjusted for confounding factors.Results in energy-adjusted analyses, the intakes of dark green and deep yellow vegetables and fruits (partial R(2) = 4.8%), total fruits and juices (partial R(2) = 1.8%), vegetables and fruits (partial R(2) = 1.8%), carrots (partial R(2) = 1.4%) and citrus fruits and juices only (partial R(2) = 0.8%) were positively correlated only with serum total carotene levels, after adjusting for serum total cholesterol concentration, age, hospital attended, smoking status. BMI and presence of cervical lesions Multiple-adjusted serum levels of carotenoids were positively correlated with intake quartiles of dark green and deep yellow vegetables and fruits and total fruits and juices independent of smoking status.Conclusions the intake of specific fruits and vegetables was an independent predictor of serum total carotene levels in low-income women living in São Paulo
- ItemAcesso aberto (Open Access)Eficácia de um programa de educação alimentar e nutricional para o aumento do consumo de hortaliças e frutas por escolares(Universidade Federal de São Paulo (UNIFESP), 2010-07-28) Nasser, Leila Adnan [UNIFESP]; Amancio, Olga Maria Silverio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: Evaluate the effectiveness of a food and nutrition educational program in the increase of the consumption of vegetables by school children. Methods: This is a longitudinal randomized controlled study performed in 280 (two hundred and eighty) children, of both genders, aged between 11 (eleven) and 12 (twelve) years old, students of 03 (three) fundamental education schools of São Paulo city, from February to September 2007. At first, all students answered the Food Frequency Questionnaire (FFQ) and than were divided randomly in two different groups: intervention and control group. After that, the intervention group was submitted to a Food and Nutritional Education Program based on ludo pedagogical activities. After 05 (five) months, the Food Frequency Questionnaire (FFQ) was applied again, in order to verify the effectiveness of the food and nutritional education program in increasing the consumption of vegetables. Results: It was observed that in the interventional group, an increase of the consumption of vegetables was significantly bigger than in the control group, indicating that the ludo pedagogical activities may have influenced the children’s eating habit in a positive way. In the control group there were no significant changes. Regarding school children that didn’t have the habit of consuming vegetables, it was observed that in the intervention group the introduction of the consumption of different kinds of vegetable (chard/cabbage, beet, avocado, papaya, mango and strawberry), was greater then in the control group, indicating that the educational intervention method may also have influenced this change. Conclusion: The food and nutrition education based on ludo pedagogical activities is effective in relation to vegetables, both for increasing the previously consumed amount and for the acceptance of different vegetables by school children.
- ItemSomente MetadadadosIsolation of bacteriocinogenic strain of Lactococcus lactis subsp lactis from rocket salad (Eruca sativa Mill.) and evidences of production of a variant of nisin with modification in the leader-peptide(Elsevier B.V., 2013-10-01) Kruger, Monika Francisca; Barbosa, Matheus de Souza; Miranda, Antonio [UNIFESP]; Landgraf, Mariza; Destro, Maria Teresa; Todorov, Svetoslav Dimitrov; Gombossy de Melo Franco, Bernadette Dora; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)In this study we have isolated and identified strains of lactic acid bacteria (LAB) that are potential bacteriocin producers from rocket salad samples acquired in São Paulo, SP, Brazil. Using the PCR-ARDRA method, Lactococcus lactis subsp. lactis MKO2R was identified and characterized as the only bacter-iocinogenic among 12 strains initially isolated. the analysis of the antimicrobial peptide produced by MK02R revealed the presence of nisin variant, a well-known lantibiotic, and one of the most studied and important antimicrobial peptides known today. the bacteriocin MK02R showed heat stability for 1 h at 60 degrees C and 100 degrees C and it was inactivated by treatment with proteolytic enzymes. the antimicrobial activity did not change with pH adjustment between 2.0 and 9.0 and the production was stimulated by the addition of 0.5 and 1.0% cysteine in MRS broth at 37 degrees C. Bacteriocin MK0R inhibited the growth of Enterococcus faecium, Lactobacillus sakei, Lactobacillus del brueckii, Lb. sakei subsp. sakei, Listeria innocua and Listeria monocytogenes from different serological groups. Low levels of adsorption of bacteriocin MK0R were detected in the cell surface of the producer cells suggesting that bacteriocin MK0R remains bound to the outer surface and that it is released when the pH of the environment increases. the chromatographic studies and the genetic tests using primers nisF and nisR related to specific genes of nisin production confirmed that the antimicrobial MKO2R is a natural variant of nisin. the partial sequencing of the reconstructed protein showed that the peptide has an amino acid change in the sequence of the leader peptide compared with nisin A, Z, Q U and F, but the structure of the mature is homologous to that of nisin F. (C) 2013 Elsevier B.V. All rights reserved.