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- ItemAcesso aberto (Open Access)Aspectos histológicos de fragmentos esplênicos autotransplantados em ratos(Universidade Federal de Minas Gerais, Escola de Veterinária, 2010-06-01) Freitas, Silvio Henrique; Dória, Renata Gebara Sampaio; Mendonça, Fábio de Souza; Evêncio Neto, J.; Simões, Manuel de Jesus [UNIFESP]; Camargo, Lázaro Manoel; UNIC Faculdade de Medicina Veterinária; Universidade Federal Rural de Pernambuco; Universidade Federal de São Paulo (UNIFESP)The splenic tissue autograft viability (one third of the spleen) associated to parcial splenectomy was studied to verify the evolutive kinetic of its regeneration by macroscopic and histological aspects. Twenty-eight adult male Wistar rats, weighting 300g, were distributed in four experimental groups. Each animal was submitted to parcial splenectomy and one fragment of each spleen was autotransplanted to the greater omentum, for a period of 30, 60, 90, and 120 days. After each period, the autotransplanted splenic tissues were collected and histopathologically analyzed. The results showed regeneration of the splenic capsule, blood vessels, white pulp, and red pulp. After 90 days, the microscopic architecture was similar to the normal spleen.
- ItemAcesso aberto (Open Access)Aspectos morfológicos, morfométricos e ultraestruturais do baço de ratos após o clampeamento total do pedículo hepático(Universidade Federal de Minas Gerais, Escola de Veterinária, 2009-12-01) Freitas, Silvio Henrique; Evêncio Neto, Joaquim; Dória, Renata Gebara Sampaio; Mendonça, Fábio de Souza; Simões, Manuel de Jesus [UNIFESP]; Camargo, Lázaro Manoel; Sebe, A.a.; UNIC Faculdade de Medicina Veterinária; Universidade Federal Rural de Pernambuco; Universidade de Federal de São Paulo; UNIC Faculdade de MedicinaThe macro and microscopic alterations that occurred in the spleen during an ischemia produced by the hepatic pedicle total clamping were studied. Forty male rats were distributed in four groups of 10 animals each. The control group (C) was not submitted to ischemia and the treated groups (E1, E2, and E3) were submitted to the clamping during 10, 20, and 30 minutes, respectively. Spleen fragments were collected and histologically analyzed by the light microscopy (eosin-hematoxilin and ferric ferrocyanide) and by the transmission electron microscopy. The results showed that 10 minutes of hepatic pedicle total clamping was enough produce signs of splenic congestion and 20 and 30 minutes promoted intense red bood cels digestion by the macrophages with the presence of iron granules (hemosiderin) in the splenic parenchyma.
- ItemSomente MetadadadosEfeitos das experiências adversas precoces e tardias no comportamento alimentar e morfologia da glândula adrenal, fígado e baço de ratos sprague-dawley submetidos ao estresse na vida adulta(Universidade Federal de São Paulo (UNIFESP), 2016-11-29) Pastene, Belgica Katuscha Vasquez [UNIFESP]; Smith, Ricardo Luiz Smith [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Early interruption of maternal bonding and social isolation are variables involved in social and emotional behavior, which can cause increased anxiety, especially in stressful situations. However, there is no research to explain the phenomenon of overeating anxiety disorder associated with these factors in an animal model and their effects on the morphology of the adrenal gland (AG), liver and spleen. Henceforth, the objective of this research was to determine the effects of early and late intake behavior in adrenal gland, liver and spleen morphology of Sprague-Dawley rats subjected to chronic stress during adulthood. We used 35 newborn female rats, maintained under standardized suckling and feeding conditions. The rats were randomized into 2 control groups (C1 and C2) and 3 experimental groups exposed to an early adverse experience (E1), late (E2) and early-late (E3) subsequently subjected to chronic intermittent stress in adulthood (C2, E1, E2 and E3). Intake behavior of groups and morphological changes of the left AG, the liver and spleen were evaluated by determining morphometric and stereological parameters for each organ. The early and late adverse experiences are factors that influence coping with a stressful situation favoring increased caloric intake and palatable food choice. The morphological features of the AG, the liver and spleen of rats can be affected by exposure to chronic stress, however, when individuals have undergone previous adverse experiences, these changes are more obvious.
- ItemSomente MetadadadosProposta de sistematização do tratamento não operatório das lesões traumáticas do baço no hospital universitário da UNIFESP/EPM(Universidade Federal de São Paulo (UNIFESP), 2015-05-27) Carlotto, Jorge Roberto Marcante [UNIFESP]; Colleoni Neto, Ramiro Colleoni Neto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objetivo: elaborar um protocolo de tratamento não operatório das lesões traumáticas do baço para ser utilizado no Hospital São Paulo - Hospital Universitário da UNIFESP/EPM.