Navegando por Palavras-chave "carbapenemase"

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    Clinical and microbiological characterization of KPC-producing Klebsiella pneumoniae infections in Brazil
    (Brazilian Society of Infectious Diseases, 2011-02-01) Beirão, Elisa Maria; Furtado, Juvencio Jose Duailibe; Girardello, Raquel [UNIFESP]; Ferreira Filho, Heli; Gales, Ana Cristina [UNIFESP]; Hospital Heliópolis; Hospital Heliópolis Department of Infectious Diseases; Universidade Federal de São Paulo (UNIFESP); Hospital Heliópolis Microbiology Laboratory
    In 2008 isolates of KPC-producing Klebsiella pneumoniae (KPC-KPN) were detected for the first time at Hospital Heliópolis, São Paulo, Brazil. The aim of this study was to characterize the clinical and microbiological outcomes of infections caused by KPC-KPN. A historical cohort of patients from whom KPC-KPN strains were isolated was performed. Isolates were identified as resistant to ertapenem by automated broth microdilution system and screened as carbapenemase producers by the modified Hodge test. The beta-lactamase resistance gene blaKPC was detected by PCR. The genetic relatedness of isolates was determined by PFGE. The study provides early clinical experience in treating KPC-KPN infections in a Brazilian tertiary center.
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    Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
    (Frontiers Media Sa, 2016) Nascimento, Ana P. B.; Ortiz, Mauro F.; Martins, Willames Marcos Brasileiro da Silva [UNIFESP]; Morais, Guilherme L.; Fehlberg, Lorena Cristina Corrêa [UNIFESP]; Almeida, Luiz G. P.; Ciapina, Luciane P.; Gales, Ana Cristina [UNIFESP]; Vasconcelos, Ana T. R.
    Carbapenems represent the mainstay therapy for the treatment of serious P aeruginosa infections. However, the emergence of carbapenem resistance has jeopardized the clinical use of this important class of compounds. The production of SPM-1 metallo-beta-lactamase has been the most common mechanism of carbapenem resistance identified in P. aeruginosa isolated from Brazilian medical centers. Interestingly, a single SPM-1-producing P. aeruginosa clone belonging to the ST277 has been widely spread within the Brazilian territory. In the current study, we performed a next-generation sequencing of six SPM-1-producing P. aeruginosa ST277 isolates. The core genome contains 5899 coding genes relative to the reference strain P. aeruginosa PAO1. A total of 26 genomic islands were detected in these isolates. We identified remarkable elements inside these genomic islands, such as copies of the bla(spM-1) gene conferring resistance to carbapenems and a type I-C CRISPR-Cas system, which is involved in protection of the chromosome against foreign DNA. In addition, we identified single nucleotide polymorphisms causing amino acid changes in antimicrobial resistance and virulence-related genes. Together,these factors could contribute to the marked resistance and persistence of the SPM-1-producing P aeruginosa ST277 clone. A comparison of the SPM-1-producing P. aeruginosa ST277 genomes showed that their core genome has a high level nucleotide similarity and synteny conservation. The variability observed was mainly due to acquisition of genomic islands carrying several antibiotic resistance genes.