Navegando por Palavras-chave "chromosomes"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosChromosome analysis of five Brazilian species of poison frogs (Anura: Dendrobatidae)(Indian Acad Sciences, 2011-04-01) Rodrigues, Paula Camargo; Aguiar, Odair [UNIFESP]; Serpieri, Flavia; Lima, Albertina Pimentel; Uetanebaro, Masao; Recco-Pimentel, Shirlei Maria; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de São Paulo (UNIFESP); Inst Nacl Pesquisas Amazonas; Universidade Federal de Mato Grosso do Sul (UFMS)Dendrobatid frogs have undergone an extensive systematic reorganization based on recent molecular findings. The present work describes karyotypes of the Brazilian species Adelphobates castaneoticus, A. quinquevittatus, Ameerega picta, A. galactonotus and Dendrobates tinctorius which were compared to each other and with previously described related species. All karyotypes consisted of 2n = 18 chromosomes, except for A. picta which had 2n = 24. The karyotypes of the Adelphobates and D. tinctorius species were highly similar to each other and to the other 2n = 18 previously studied species, revealing conserved karyotypic characteristics in both genera. In recent phylogenetic studies, all Adelphobates species were grouped in a clade separated from the Dendrobates species. Thus, we hypothesized that their common karyotypic traits may have a distinct origin by chromosome rearrangements and mutations. In A. picta, with 2n = 24, chromosome features of pairs from 1 to 8 are shared with other previously karyotyped species within this genus. Hence, the A. picta data reinforced that the C-banding pattern and the NOR location are species-specific traits in the genus Ameerega. Moreover, the Ameerega monophyletism proposed by previous phylogenetic studies indicates that the karyotypic differences among species in this genus result from a long divergence time.
- ItemAcesso aberto (Open Access)Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias(Associação Paulista de Medicina - APM, 1997-02-01) Chauffaille, Maria de Lourdes Lopes Ferrari [UNIFESP]; Coutinho, Vicente [UNIFESP]; Yamamoto, Mihoko [UNIFESP]; Kerbauy, José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)In the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology, citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb: in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present.
- ItemAcesso aberto (Open Access)Isolated trisomy 11 in de novo acute myeloid leukemia(Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, 2008-01-01) Krum, Everson A. [UNIFESP]; Hamed, Tatiane [UNIFESP]; Yamamoto, Mihoko [UNIFESP]; Chauffaille, Maria de Lourdes Lopes Ferrari [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The real mechanism involved in trisomies and leukemogenesis remains unknown and more information about this connection is essential, but unfortunately the clinical outcome and hematological profile of patients with isolated trisomy 11 and AML have not been well characterized. Considering the limited data on the specific disease entity, the current report describes two cases of de novo acute monocytic leukemia (AMoL) and isolated +11, in which this event was further characterized.
- ItemSomente MetadadadosSchizophrenia and fragile sites(Lippincott Williams & Wilkins, 1997-12-01) Smith, Marilia de Arruda Cardoso [UNIFESP]; Souza, Mônica Aparecida Valentim de [UNIFESP]; Payão, Spencer Luiz Marques [UNIFESP]; Pugliese, Salvador; Mari, Jair de Jesus [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)