Navegando por Palavras-chave "colitis"

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    Efeitos da suplementação de vitamina d3 na colite experimental: avaliação imunológica e histológica
    (Universidade Federal de São Paulo (UNIFESP), 2015-07-24) Souza, Marilia Graziela Alves de [UNIFESP]; Sdepanian, Vera Lucia Sdepanian [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: The objective of this study was to evaluate the influence of vitamin D3 supplementation in acute colitis, induced bu DSS, in the cytokines, in shortening and histology of the colon. Method: Sixty Wistar rats were divided into four groups: untreated colitis, treated with Vitamin D3 (100 IU/day), treated with mesalamine (50 mg/kg/day) and control. All of the animals went under induction of colitis by DSS for 5 days, except the control group. From serum and colon, obtained after euthanasia one day or four days after the end of the DSS, were quantified cytokinem size and weight of the colon and histological evaluation. Results: The vitamin D3 did not alter the concentration of the studied pro and antii-inflammatory cytokines. Regarding colon shortening, in the acute immediate time, the group trated with vitamin D3 presented shortening as well as untreated colitis group, however they were able to recover their length four days after the DSS induction. Regarding histology, the group treated with vitamin D3 presented a lower proportion of histological damage in comparison to the untreated colitis groups and colitis treated with mesalamine. Conclusion: Vitamin D3 supplementation in rats under to colitis induced by DSS was unable to modulate the concentrations of cytokines, but could promote the recovery of colon size four days after colitis induction, and lower histological damage than the untreated colitis groups and the treated with mesalamine group, therefore optimizing tissue healing. .
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    Partial Replacement of omega-6 Fatty Acids With Medium-Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis
    (Sage Publications Inc, 2012-07-01) Bertevello, Pedro L.; De Nardi, Leticia; Torrinhas, Raquel S.; Logullo, Angela F. [UNIFESP]; Waitzberg, Dan L.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Background: Soybean oil is rich in omega-6 fatty acids, which are associated with higher incidence and more severe cases of inflammatory bowel diseases. the authors evaluated whether partial replacement of soybean oil by medium-chain triglycerides (MCTs) or olive oil influenced the incidence and severity of experimental ulcerative colitis by using different parenteral lipid emulsions (LEs). Methods: Wistar rats (n = 40) were randomized to receive parenteral infusion of the following LE: 100% soybean oil (SO), 50% MCT mixed with 50% soybean oil (MCT/SO), 80% olive oil mixed with 20% soybean oil (OO/SO), or saline (CC). After 72 hours of infusion, acetic acid experimental colitis was induced. After 24 hours, colon histology and cytokine expression were analyzed. Results: SO was not significantly associated with overall tissue damage. MCT/SO was not associated with necrosis (P < .005), whereas OO/SO had higher frequencies of ulcer and necrosis (P < .005). SO was associated with increased expression of interferon-gamma (P = .005) and OO/SO with increased interleukin (IL)-6 and decreased tumor necrosis factor-alpha expression (P < .05). MCT/SO appeared to decrease IL-1 (P < .05) and increase IL-4 (P < .001) expression. Conclusions: Parenteral SO with high concentration of omega-6 fatty acids was not associated with greater tissue damage in experimental colitis. SO partial replacement with MCT/SO decreased the frequency of histological necrosis and favorably modulated cytokine expression in the colon; however, replacement with OO/SO had unfavorable effects. (JPEN J Parenter Enteral Nutr. 2012; 36: 442-448)
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    The relevance of kinin B(1) receptor upregulation in a mouse model of colitis
    (Wiley-Blackwell, 2008-07-01) Hara, D. B.; Leite, D. F. P.; Fernandes, E. S.; Passos, G. F.; Guimaraes, Alessander de Oliveira [UNIFESP]; Pesquero, João Bosco [UNIFESP]; Campos, M. M.; Calixto, J. B.; Universidade Federal de Santa Catarina (UFSC); Universidade Federal de São Paulo (UNIFESP); Pontificia Univ Catolica Rio Grande do Sul
    Background and purpose: Kinins are implicated in many pathophysiological conditions, and recent evidence has suggested their involvement in colitis. This study assessed the role of the kinin B(1) receptors in a mouse model of colitis.Experimental approach: Colitis was induced in mice by 2,4,6-trinitrobenzene sulphonic acid (TNBS), and tissue damage and myeloperoxidase activity were assessed. B(1) receptor induction was analysed by organ bath studies, binding assay and reverse transcription PCR.Key results: TNBS-induced colitis was associated with tissue damage, neutrophil infiltration and time-dependent increase of colon B(1) receptor-mediated contraction, with the maximal response observed at 72 h. the upregulation of the B(1) receptor at this time point was also confirmed by means of binding studies. B(1) receptor mRNA levels were elevated as early as 6 h after colitis induction and remained high for up to 48 h. TNBS-evoked tissue damage and neutrophil influx were reduced by the selective B(1) receptor antagonist SSR240612, and in B(1) receptor knockout mice. in vivo treatment with inhibitors of protein synthesis, nuclear factor-kappa B activation, inducible nitric oxide synthase (iNOS) or tumour necrosis factor alpha (TNF alpha) significantly reduced B(1) receptor agonist-induced contraction. Similar results were observed in iNOS and TNF receptor 1-knockout mice.Conclusions and implications: These results provide convincing evidence on the role of B1 receptors in the pathogenesis of colitis. Therefore, the blockade of kinin B1 receptors might represent a new therapeutic option for treating inflammatory bowel diseases.
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    Risk factors for low bone mineral density in children and adolescents with inflammatory bowel disease
    (Springer, 2008-10-01) Caldas Lopes, Leticia Helena [UNIFESP]; Sdepanian, Vera Lucia [UNIFESP]; Szejnfeld, Vera Lucia [UNIFESP]; Morais, Mauro Batista de [UNIFESP]; Fagundes-Neto, Ulysses [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective To evaluate bone mineral density of the lumbar spine in children and adolescents with inflammatory bowel disease, and to identify the clinical risk factors associated with low bone mineral density. Methods Bone mineral density of the lumbar spine was evaluated using dual-energy X-ray absorptiometry (DXA) in 40 patients with inflammatory bowel disease. Patients were 11.8 (SD = 4.1) years old and most of them were male (52.5%). Multiple linear regression analysis was performed to identify potential associations between bone mineral density Z-score and age, height-for-age Z-score, BMI Z-score, cumulative corticosteroid dose in milligrams and in milligrams per kilogram, disease duration, number of relapses, and calcium intake according to the dietary reference intake. Results Low bone mineral density (Z-score bellow -2) was observed in 25% of patients. Patients with Crohn's disease and ulcerative colitis had equivalent prevalence of low bone mineral density. Multiple linear regression models demonstrated that height-for-age Z-score, BMI Z-score, and cumulative corticosteroid dose in mg had independent effects on BMD, respectively, beta = 0.492 (P = 0.000), beta = 0.460 (P = 0.001), beta = -0.014 (P = 0.000), and these effects remained significant after adjustments for disease duration, respectively, beta = 0.489 (P = 0.013), beta = 0.467 (P = 0.001), and beta = -0.005 (P = 0.015). the model accounted for 54.6% of the variability of the BMD Z-score (adjusted R(2) = 0.546). Conclusions the prevalence of low bone mineral density in children and adolescents with inflammatory bowel disease is considerably high and independent risk factors associated with bone mineral density are corticosteroid cumulative dose in milligrams, height-for-age Z-score, and BMI Z-score.