Navegando por Palavras-chave "disfunção erétil"
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- ItemAcesso aberto (Open Access)Daily use of phosphodiesterase type 5 inhibitors as prevention for recurrent priapism(Assoc Medica Brasileira, 2017) Nardozza Junior, Archimedes [UNIFESP]; Cabrini, Marcelo Rodrigues [UNIFESP]Objective: The pathogenesis of recurrent priapism is currently being investigated based on the regulation of the phosphodiesterase 5 (PDE5) enzyme. We explored the daily use of PDE5 inhibitors to treat and prevent priapism recurrences. Method: We administered PDE5 inhibitors using a long-term therapeutic regimen in seven men with recurrent priapism, with a mean age of 29.2 years (range 21 to 35 years). Six men (85.7%) had idiopathic priapism recurrences and one man (24.3%) had sickle cell disease-associated priapism recurrences. Tadalafil 5 mg was administered daily. The mean follow-up was 6.6 months (range 3 to 12 months). Results: Daily long-term oral PDE5 inhibitor therapy alleviated priapism recurrences in all patients. Five (71.4%) had no episodes of priapism and two (28.6%) referred decrease in their episodes of priapism. All patients referred improvement in erectile function. Conclusion: These findings suggest the hypothesis that PDE5 dysregulation exerts a pathogenic role for both sickle cell disease-associated priapism and for idiopathic priapism, and that it offers a molecular target for the therapeutic management of priapism. These preliminary observations suggest that continuous long-term oral PDE5 inhibitor therapy may treat and prevent recurrent priapism.
- ItemSomente MetadadadosEstudo da relação estrutura-atividade de fragmentos da atx, uma proteína extraída do veneno da latrodectus mactans do chile(Universidade Federal de São Paulo (UNIFESP), 2013-12-20) Silva, Thalita Melo Teixeira Porto da [UNIFESP]; Miranda, Antonio de Miranda [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Arachnotoxin (ATx) é um potente polipeptídeo com propriedades eretógenas isolado./ a partir do veneno da aranha Latrodectus Chile. Neste trabalho a síntese e as propriedades biológicas dos dois fraqrne.rtos de ATX, uma linear e outra cíclica, foram descritas de forma a desenvolver um estudo de relação estrutura-atividade. Assim, os peptídeos foram obtidos pelo método de síntese de fase sólida pela estratégia t-Boc, em seguida os análogos foram clivados, purificados (CLAE) e caracterizados por CLlEM-IES e AAA. A análise de estrutura secundária foi feita pela técnica de dicroísmo circular (CO), onde foram testadas a influência do pH, SOS (1 e 15 mM), e TFE (10, 30, 50 e 70%) e de H20 na conformação dos fragmentos. A estabilidade dos derivados do ATx foi estudada em enzimas totais de lisado celular. Observamos um comportamento conformacional semelhante dos fragmentos quando analisados por CO quanto a variação do pH, onde todos apresentaram um tendência de estrutura randômica. Já em SOS todos os fragmentos apresentaram uma tendência de conformação em alfa-hélice, assim como na presença de TFE. Finalmente, em água todos os peptídeos também apresentaram uma estrutura randômica. Nos estudos com as enzimas totais de lisado celular a estabilidade dos fragmentos foram preservadas num período de incubação de até 120 minutos.
- ItemSomente MetadadadosExpressão de fatores indutores de fibrose no tecido cavernoso de pacientes com disfunção erétil pós prostatectomia radical(Universidade Federal de São Paulo (UNIFESP), 2015-12-15) Cabrini, Marcelo Rodrigues [UNIFESP]; Ribeiro, Cassio Andreoni Ribeiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objectives: To characterize transforming growth factor beta 1 (TGF?1) and related signaling pathway proteins in a large cohort of human penile tissue (HPT) samples from men with post-radical prostatectomy (RP) erectile dysfunction (ED). Method: HPT was collected from patients undergoing penile prosthesis implantation for postradical prostatectomy erectile dysfunction (RP-ED; n = 57). Two groups of tissue were used for comparison: organic ED (O-ED; n = 30), also collected during penile prosthesis implantation, and control (CON; n = 6), collected from patients undergoing partial penectomy, without ED. Western blot analysis was performed to investigate the protein expressions of TGF?1, thrombospondin 1 (TSP1; an activator of TGF?1), a family of transcriptional factors activated by TGF?1 (Smad2, phospho-Smad2- serine-465/467 [pSmad2], Smad3, phospho-Smad3-serine-423/425 [pSmad3]), and fibronectin (FN; an extracellular matrix glycoprotein induced by TGF?1). Results: Expressions of TGF?1 and TSP1 were significantly higher in RP-ED (p <0.05) and OED (p <0.05) groups compared with that of the CON group and were not different between either ED groups. Expressions of Smad2, pSmad2, Smad3, pSmad3, and FN were similar among all groups. Within the RP-ED group, a subgroup analysis showed that body mass index (BMI)>25Kg/m2 was related to increased expression of TGF?1 and TSP1, previous history of intracavernosal injection was related to increased expression of TGF?1 (p<0.05), and time from RP to penile prosthesis implantation (moment of tissue collection) was related to increased expression of pSmad2 (p<0.05). Conclusions: Our results demonstrate that TSP1- and TGF?1- dependent fibrotic changes occur in penile tissue in patients with ED regardless of etiology. The unchanged expression of the Smad transcriptional factors may be reconciled by a Smad-independent downstream signaling pathway transmitting TGF?1 signals. Among RP-ED men, BMI, previous use of intracavernosal injection and time sice RP affect fibrotic protein expression.