Navegando por Palavras-chave "ensaio de alta sensibilidade"

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    Development, characterization and clinical validation of new sensitive immunofluorometric assay for the measurement of serum thyroglobulin
    (Sociedade Brasileira de Endocrinologia e Metabologia, 2012-12-01) Nakabashi, Cláudia C. D. [UNIFESP]; Biscolla, Rosa Paula M. [UNIFESP]; Kasamatsu, Teresa S. [UNIFESP]; Tachibana, Teresinha T.; Barcelos, Rafaela N. [UNIFESP]; Malouf, Eduardo Z.; Andreoni, Danielle M. [UNIFESP]; Maciel, Rui Monteiro de Barros [UNIFESP]; Vieira, José Gilberto H. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    OBJECTIVE: In the last decade, data published stressed the role of highly-sensitive thyroglobulin (Tg) assays in the follow-up of differentiated thyroid carcinoma (DTC) patients. The present study describes a new, highly-sensitive Tg assay, compares it with an available commercial assay, and validates it in the follow-up of DTC patients. SUBJECTS AND METHODS: The immunofluorometric high-sensitivity Tg assay is based on monoclonal and polyclonal antibodies produced at our laboratories. It was validated in 100 samples of 87 patients with DTC submitted to total thyroidectomy, 87% of whom also received radioiodine. For correlation, all samples were also tested using a commercial Tg assay (Beckman Access) with functional sensitivity (FS) of 0.1 ng/mL. RESULTS: The new method showed FS of 0.3 ng/mL. The correlation between the two methods was good (r = 0.74; p < 0.0001). The diagnostic sensitivity was 88.9%, and it was increased to 100% when combined with neck US. CONCLUSION: This new, high-sensitivity Tg assay presented a good correlation with Beckman Access assay and with the clinical outcome of the patients. The continuous availability of a validated assay is an additional advantage for long term follow-up of DTC patients. Arq Bras Endocrinol Metab. 2012;56(9):658-65