Navegando por Palavras-chave "fator de crescimento do endotélio vascular derivado de glândula endócrina"

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    Avaliação de marcadores de receptividade endometrial em modelos experimentais de ratas com anovulação
    (Universidade Federal de São Paulo (UNIFESP), 2014-11-28) Tarasconi, Bruno Ventura [UNIFESP]; Motta, Eduardo Leme Alves da Motta [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: The aim of the study was to assess endometrial histomorphometric features and cytokines expression involved in embryo implantation in the endometrium of female rats with anovulatory cycles induced by continuous illumination in comparison to control rats, with ovulatory cycles, maintained in intermittent light exposure. Methods: We have studied 27 Wistar-Albino female rats that were divided into two groups: experimental (14 rats under continuous illumination with subsequent onset of anovulatory cycles) and control (13 ovulatory rats maintained in clear/dark cycle of 12/12 hours). After colpocytologic monitoring of the estrus cycle phases, animals were submitted to euthanasia and hysterectomy at the right moment: pro-estrus phase for the control group; estrus phase for the experimental group. The resulting uteri were evaluated for four histomorphometric parameters: endometrial thickness, myometrial thickness, vascular surface and glandular surface and two biomolecular parameters: concentration of LIF and PROKR1, evaluated by ELISA sandwich. Results: The endometrial thickness, myometrial thickness and vascular surface have shown to be greater in the anovulatory rats group, exposed to continuous illumination (p<0,05), while the glandular surface, also greater in such group, has demonstrated a trend to statistical significance (p=0,096). Inversely, the biomolecular parameters, represented by concentrations of LIF and PROKR1, have shown to be the opposite, with smaller concentrations in the experimental group, represented by anovulatory rats (p<0,05). Conclusions: The endometrial histomorphometric features of female anovulatory rats, although suitable considering the structural point of view, do not mean favorable functional state for implantation. Anovulation is associated with the impairment of endometrial receptivity since it leads to the deficiency of LIF and PROKR1 production. The continuous light exposure certainly influences the reproductive potencial in rats, as it establishes anovulation and determines impairment to embryonic receptiveness.