Navegando por Palavras-chave "fibroblast"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosAffected and non-affected skin fibroblasts from systemic sclerosis patients share a gene expression profile deviated from the one observed in healthy individuals(Clinical & Exper Rheumatology, 2008-09-01) Fuzii, Hellen Thais [UNIFESP]; Yoshikawa, Gilberto Toshimitsu [UNIFESP]; Junta, C. M.; Sandrin-Garcia, P.; Fachin, A. L.; Sakamoto-Hojo, E. T.; Donadi, E. A.; Passos, G. A. S.; Andrade, Luiz Eduardo Coelho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)ObjectivesTo evaluate the gene expression profile of fibroblasts from affected and non-affected skin of systemic sclerosis (SSc) patients and from controls.Materials and methodsLabeled cDNA from fibroblast cultures from forearm (affected) and axillary (non-affected) skin from six diffuse SSc patients, from three normal controls, and from MOLT-4/HEp-2/normal fibroblasts (reference pool) was probed in microarrays generated with 4193 human cDNAs from the IMAGE Consortium. Microarray images were converted into numerical data and gene expression was calculated as the ratio between fibroblast cDNA (Cy5) and reference pool cDNA (Cy3) data and analyzed by R environment/Aroma, Cluster, Tree View, and SAM softwares. Differential expression was confirmed by real time PCR for a set of selected genes.ResultsEighty-eight genes were up- and 241 genes down-regulated in SSc fibroblasts. Gene expression correlation was strong between affected and non-affected fibroblast samples from the same patient (r>0.8), moderate among fibroblasts from all patients (r=0.72) and among fibroblasts from all controls (r=0.70), and modest among fibroblasts from patients and controls (r=0.55). The differential expression was confirmed by real time PCR for all selected genes.ConclusionsFibroblasts from affected and non-affected skin of SSc patients shared a similar abnormal gene expression profile, suggesting that the widespread molecular disturbance in SSc fibroblasts is more sensitive than histological and clinical alterations. Novel molecular elements potentially involved in SSc pathogenesis were identified.
- ItemAcesso aberto (Open Access)Development of experimental in vitro burn model(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Fernandes, Ana Carolina Morais; França, Jerônimo Pereira de [UNIFESP]; Gaiba, Silvana; Aloise, Antonio Carlos [UNIFESP]; Oliveira, Andrea Fernandes de; Moraes, Andrea Aparecida Fátima Souza [UNIFESP]; França, Lucimar Pereira de; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Santa Cruz Department of Biological SciencesPURPOSE:To propose an experimental burn model in NIH-3T3 cell line.METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
- ItemSomente MetadadadosDoes Preoperative Electrical Stimulation of the Skin Alter the Healing Process?(Elsevier B.V., 2011-04-01) Borba, Graziela Maria Chacon [UNIFESP]; Hochman, Bernardo [UNIFESP]; Liebano, Richard E.; Enokihara, Milvia M. S. S. [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ City São Paulo UNICIDBackground. in vitro studies have demonstrated that electrical current may affect fibroblast proliferation and synthesis of collagen fibers. in humans, the application of electrical current by positioning the positive electrode on skin wounds resulted in thinner hypertrophic scars. the aim of this study was to evaluate the effects of preoperative electrical stimulation on cutaneous wound healing in rats.Materials and Methods. Forty rats were divided into two groups of 20 animals each. in the control group, an incision was made on the back of the animals. in the stimulation group, a preoperative electrical stimulation was applied using a rectangular pulse current at a frequency of 7.7 Hz, and intensity of 8 mA, for 30 min, with the positive electrode placed on the back of the animal, and the negative electrode placed on the abdominal wall. Following, an incision was made on their back. Biopsy was carried out on postoperative day 7 and 14, and histologic analysis was performed.Results. the number of newly formed vessels, fibroblasts, and type III collagen fibers in the stimulation group on postoperative day 7 were greater than those in the control group.Conclusions. Preoperative positive-polarity electrical stimulation positively affects angiogenesis and fibroblast proliferation. (C) 2011 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosGap junctions between mast cells and fibroblasts in the developing avian eye(Karger, 1995-01-01) Oliani, Sonia Maria [UNIFESP]; Girol, Ana Paula [UNIFESP]; Smith, Ricardo Luiz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Mast cells are present in the eye of chick embryos from the 14th day onward, displaying metachromatic granules, mainly in the iris anterior surface and pectinate ligament, Ultrastructurally these cells show electron-dense granules and a few thin and short cytoplasmic projections in close contact with fibroblasts. Sometimes these contacts are extensive, with long fibroblast projections partially involving the mast cells. Gap junctions between mast cells and fibroblasts are observed only in the eyes of 16- and 20-day-old embryos. These intercellular specializations are represented by a close apposition of cytoplasmic membranes with an extension up to 300 nm. Gap junctions between mast cells and fibroblasts were not observed previously in vivo or in vitro, although in vitro studies have shown that a number of functionally critical interactions may occur between these cells. Our morphological findings suggest that, in vivo, fibroblasts interact with mast cells and may influence their maturation.