Navegando por Palavras-chave "gingiva"

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    Cimetidine Reduces Alveolar Bone Loss in Induced Periodontitis in Rat Molars
    (Amer Acad Periodontology, 2014-08-01) Longhini, Renata [UNIFESP]; Oliveira, Priscila Aparecida de [UNIFESP]; Sasso-Cerri, Estela; Cerri, Paulo Sergio; Universidade Federal de São Paulo (UNIFESP); São Paulo State Univ
    Background: There is evidence that histamine released during inflammation plays a role in bone metabolism via the H-2 receptor, stimulating bone resorption. the purpose of this study is to evaluate whether cimetidine, a histamine H-2-receptor antagonist, interferes with the initiation and progression of induced periodontal disease in rat molars.Methods: Forty male rats received 100 mg/kg body weight of cimetidine (cimetidine group [CimG]) or saline solution (sham group [SG]). Periodontal disease was induced in the maxillary left first molars (PDSG and PDCimG); maxillary right molars were used as non-ligature controls. After 7, 15, 30, and 50 days, maxillary fragments were embedded in paraffin. the sections were stained with Masson trichrome and hematoxylin and eosin and subjected to the tartrate-resistant acid phosphatase (TRAP) method. the distances between the cemento-enamel junction (CEJ) and alveolar process (AP) crest, as well as between the CEJ and junctional epithelium (JE) level, were measured; the number of inflammatory cells was computed. Receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) immunohistochemistry was carried out, and the RANKL/OPG ratio was calculated.Results: in PDSG and PDCimG, a significant increase (P <= 0.05) was observed in CEJ-AP and CEJ-JE distances. However, the increases in both distances were significantly less in PDCimG compared with PDSG at 15, 30, and 50 days. Numerous TRAP-positive osteoclasts were found in the PDSG and PDCimG. in PDCimG, the volume density of inflammatory cells and the RANKL/OPG ratio were significantly lower (P <= 0.05) than in PDSG.Conclusions: Cimetidine exerts a beneficial effect on periodontal disease in rats, decreasing the RANKL/OPG ratio in gingival connective tissue and reducing alveolar bone resorption.
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    Cimetidine Reduces Interleukin-6, Matrix Metalloproteinases-1 and-9 Immunoexpression in the Gingival Mucosa of Rat Molars With Induced Periodontal Disease
    (Amer Acad Periodontology, 2017) Oliveira, Priscila Aparecida de [UNIFESP]; Pizzol Junior, Jose Paulo de [UNIFESP]; Longhini, Renata [UNIFESP; Sasso-Cerri, Estela; Cerri, Paulo Sergio
    Background: Histamine seems to act, via H-2 receptor, on inflammatory processes by stimulating interleukin (IL)-6 and matrix metalloproteinase (MMP) release. As cimetidine is an H-2 receptor antagonist, the authors hypothesize that this antiulcer drug reduces IL-6, MMP-1, andMMP-9immunoexpressioningingiva with inducedperiodontal disease (PD). Toconfirmapossible modulatory role of IL-6 on MMPs, the relationship between IL-6/MMP-1 and IL-6/MMP-9 immunoexpression was evaluated. Methods: Forty-six male rats were distributed into the cimetidine group (CimG: received daily intraperitoneal injections of 100 mg/kg of body weight of cimetidine) or the saline group (SG). PD was induced by cotton ligature around the maxillary left first molars (PDSG and PDCimG). The right molars were used as controls (SG and CimG). After 7, 15, 30, and 50 days, maxillary fragments were processed for paraffin embedding or for transmission electron microscopy. Tartrateresistant acid phosphatase (TRAP)-positive osteoclasts in the alveolar process surface and number of IL-6, MMP-1, and MMP-9-immunolabeled cells in the gingival mucosa were quantified. Statistical analyses were performed (P <= 0.05). Results: In PDSG and PDCimG, gingival mucosa exhibited few collagen fibers among numerous inflammatory cells. In PDCimG, the number of TRAP-positive osteoclasts and IL-6, MMP-1, andMMP-9-immunolabeled cells was significantly lower than in PDSG at all periods. A positive correlation between IL-6/MMP-1 and IL-6/MMP-9 was detected in PDSG and PDCimG. Conclusion: Cimetidine decreases bone loss through reduction of osteoclast number and induces reduction of IL-6, MMP-1, and MMP-9 immunoexpression, reinforcing the idea that the beneficial effect of cimetidine in PD may be due to reduction of IL-6 immunolabeling in the inflamed gingival mucosa.