Navegando por Palavras-chave "high molecular mass antigen"

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    Reactivity of antibodies from patients with acute and chronic paracoccidioidomycosis to a high molecular mass antigen from Paracoccidioides brasiliensis
    (Wiley-Blackwell, 2005-01-01) Marquez, A. S.; Vicentini, A. P.; Ono, M. A.; Watanabe, MAE; Camargo, Z. P. de; Itano, E. N.; Universidade Estadual de Londrina (UEL); Adolfo Lutz Inst; Universidade Federal de São Paulo (UNIFESP)
    Yeast forms of Paracoccidioides brasiliensis produce polydispersed high molecular mass (h-MM) antigens. We investigated the antibodies to an h-MM antigen from P. brasiliensis by immunoblotting and ELISA in sera from paracoccidioidomycosis (PCM) patients. IgG from the sera of chronic PCIVI patients was able to recognize the h-MM antigen at a higher frequency in the cell-free antigen (CFA) (8/13) than in the somatic antigen (SA) (2/13), as assessed by immunoblotting. the CFA was fractionated by Sephadex G-200 chromatography, and fraction 17 (F17) with the h-MM antigen of approximately 366 kDa was used in ELISA to analyze specific levels of IgG and IgE. Patients with the chronic form showed significantly higher levels of IgG (P < 0.05) but not IgE (P > 0.05) to F17 by ELISA, compared to patients with the acute form or to healthy donors. in conclusion, CFA is better than SA as a source of the P. brasiliensis h-MM antigen. This study reveals a new characteristic to differentiate between the acute and chronic forms of PCM, by demonstrating a higher level of seric IgG to h-MM antigen in chronic compared to acute PCM patients.