Navegando por Palavras-chave "implantação do embrião"

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    Avaliação de marcadores de receptividade endometrial em modelos experimentais de ratas com anovulação
    (Universidade Federal de São Paulo (UNIFESP), 2014-11-28) Tarasconi, Bruno Ventura [UNIFESP]; Motta, Eduardo Leme Alves da Motta [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: The aim of the study was to assess endometrial histomorphometric features and cytokines expression involved in embryo implantation in the endometrium of female rats with anovulatory cycles induced by continuous illumination in comparison to control rats, with ovulatory cycles, maintained in intermittent light exposure. Methods: We have studied 27 Wistar-Albino female rats that were divided into two groups: experimental (14 rats under continuous illumination with subsequent onset of anovulatory cycles) and control (13 ovulatory rats maintained in clear/dark cycle of 12/12 hours). After colpocytologic monitoring of the estrus cycle phases, animals were submitted to euthanasia and hysterectomy at the right moment: pro-estrus phase for the control group; estrus phase for the experimental group. The resulting uteri were evaluated for four histomorphometric parameters: endometrial thickness, myometrial thickness, vascular surface and glandular surface and two biomolecular parameters: concentration of LIF and PROKR1, evaluated by ELISA sandwich. Results: The endometrial thickness, myometrial thickness and vascular surface have shown to be greater in the anovulatory rats group, exposed to continuous illumination (p<0,05), while the glandular surface, also greater in such group, has demonstrated a trend to statistical significance (p=0,096). Inversely, the biomolecular parameters, represented by concentrations of LIF and PROKR1, have shown to be the opposite, with smaller concentrations in the experimental group, represented by anovulatory rats (p<0,05). Conclusions: The endometrial histomorphometric features of female anovulatory rats, although suitable considering the structural point of view, do not mean favorable functional state for implantation. Anovulation is associated with the impairment of endometrial receptivity since it leads to the deficiency of LIF and PROKR1 production. The continuous light exposure certainly influences the reproductive potencial in rats, as it establishes anovulation and determines impairment to embryonic receptiveness.
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    Endométrio na janela de implantação em mulheres com síndrome dos ovários policísticos
    (Associação Médica Brasileira, 2011-12-01) Lopes, Ione Maria Ribeiro Soares [UNIFESP]; Baracat, Maria Cândida Pinheiro; Simões, Manuel de Jesus [UNIFESP]; Simões, Ricardo Santos; Baracat, Edmund Chada [UNIFESP]; Soares Júnior, José Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Piauí; Universidade de São Paulo (USP)
    The human endometrium undergoes to a complex series of prolifertive and secretory changes in each menstrual cycle and displays only a short period of receptivity, known as the window of implantation, necessary for the implantation of the blastocyst in the uterus. The implantation process occurs in a sequential manner, leading to the establishment of pregnancy. Morphofunctional changes during this period may prevent or hinder the implantation. For this reason, the study of the endometrium at this stage is important for the improvement of therapies that may interfere with the mechanisms involved in maternal-embryonic interaction. Several gynecological disorders, including polycystic ovary syndrome (PCOS), are associated with decreased fertility and uterine receptivity. In spite of recent advances in assisted reproduction techniques, allowing the selection of high quality embryos, the implantation rate remains low and has not increased enough in recent decades. This article aims at reviewing the endometrial aspects of the window of implantation in women with polycystic ovary syndrome, focusing mainly on adhesion molecules. For that purpose, we analyzed 105 articles published in journals indexed in PubMed in the last 50 years (up to May 2011). In conclusion, the endometrial receptivity seems to be the major limiting factor for the establishment of pregnancy in a large number of gynecological diseases, including PCOS, and treatment to improve implantation rates is likely to be taken towards this direction.