Navegando por Palavras-chave "interleukin-12"

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    Cervical cerclage placement decreases local levels of proinflammatory cytokines in patients with cervical insufficiency
    (Mosby-Elsevier, 2017) Monsanto, Stephany P.; Daher, Silvia [UNIFESP]; Ono, Erika [UNIFESP]; Tezotto Pendeloski, Karen Priscilla [UNIFESP]; Traina, Evelyn [UNIFESP]; Mattar, Rosiane [UNIFESP]; Tayade, Chandrakant
    BACKGROUND: Cervical insufficiency is characterized by premature, progressive dilation and shortening of the cervix during pregnancy. If left unattended, this can lead to the prolapse and rupture of the amniotic membrane, which usually results in midtrimester pregnancy loss or preterm birth. Previous studies have shown that proinflammatory cytokines such as interleukin-1 beta, interleukin-6, interleukin-8, and tumor necrosis factor alpha are up-regulated in normal parturition but are also associated with preterm birth. Studies evaluating such markers in patients with cervical insufficiency have evaluated only their diagnostic potential. Even fewer studies have studied them within the context of cerclage surgery. OBJECTIVES(S): The objective of the study was to evaluate the impact of local and systemic inflammatory markers on the pathogenesis of cervical insufficiency and the effect of cerclage surgery on the local immune microenvironment of women with cervical insufficiency. STUDY DESIGN: We recruited 28 pregnant women (12- 20 weeks' gestation) diagnosed with insufficiency and referred for cerclage surgery and 19 gestational age-matched normal pregnant women as controls. Serum and cervicovaginal fluid samples were collected before and after cerclage surgery and during a routine checkup for normal women and analyzed using a targeted 13-plex proinflammatory cytokine assay. RESULTS: Before surgery, patients with cervical insufficiency had higher levels of interleukin-1b, interleukin-6, interleukin-12, monocyte chemoattractant protein-1 and tumor necrosis factor alpha in cervicovaginal fluid compared to controls, but after surgery, these differences disappeared. No differences were found in serum of insufficiency versus control women. In patients with insufficiency, the levels of interleukin-1b, interleukin-6, interleukin-8, monocyte chemoattractant protein-1, and interferon gamma in cervicovaginal fluid declined significantly after cerclage compared with before intervention, but these changes were not detected in serum. CONCLUSION: Compared with normal women, patients with cervical insufficiency have elevated levels of proinflammatory cytokines in cervicovaginal fluid but not in serum, suggesting a dysregulation of the local immune environment. Cerclage intervention led to a significant decline in these proinflammatory cytokines, suggesting that cerclage may help reduce local inflammation in cervical insufficiency.
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    IL-12 inhibits endotoxin-induced inflammation in the eye
    (Vch Publishers Inc, 1996-05-01) Whitcup, S. M.; Rizzo, L. V.; Lai, J. C.; Hayashi, S.; Gazzinelli, R.; Chan, C. C.; NIH; Universidade Federal de São Paulo (UNIFESP); NIAID; Universidade Federal de Minas Gerais (UFMG)
    Interleukin-12 (IL-12) is a heterodimeric cytokine that induces interferon (IFN)-gamma production and an increased generation of Th 1 cells. Both IL-12 and IL-12 antagonists are being studied for the treatment of allergic reactions, autoimmune disease and malignancy. the goal of the present experiments was to examine the importance of IL-12 in endotoxin-induced ocular inflammation. the number of inflammatory cells infiltrating eyes with endotoxin-induced uveitis (EIU) was significantly increased in animals treated with intraperitoneal anti-IL-12 antibody when compared to control animals, but there was no difference in infiltrating inflammatory cells in the eyes of animals treated with IL-12 when compared to controls. in contrast, intraocular injection of IL-12 significantly inhibited the development of endotoxin-induced intraocular inflammation. the inflammatory cells were reduced in the eves of animals receiving intraocular IL-obtained from eyes with EIU showed increased levels of IFN-gamma and decreased levels of IL-6 in eyes receiving intraocular IL-12. These data show that IL-12 has an inhibitory effect on endotoxin-induced inflammation in the eye and su that IL-12 can have an immunoregulatory function in some forms of inflammatory disease.