Navegando por Palavras-chave "interleukin-6"

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    Cervical cerclage placement decreases local levels of proinflammatory cytokines in patients with cervical insufficiency
    (Mosby-Elsevier, 2017) Monsanto, Stephany P.; Daher, Silvia [UNIFESP]; Ono, Erika [UNIFESP]; Tezotto Pendeloski, Karen Priscilla [UNIFESP]; Traina, Evelyn [UNIFESP]; Mattar, Rosiane [UNIFESP]; Tayade, Chandrakant
    BACKGROUND: Cervical insufficiency is characterized by premature, progressive dilation and shortening of the cervix during pregnancy. If left unattended, this can lead to the prolapse and rupture of the amniotic membrane, which usually results in midtrimester pregnancy loss or preterm birth. Previous studies have shown that proinflammatory cytokines such as interleukin-1 beta, interleukin-6, interleukin-8, and tumor necrosis factor alpha are up-regulated in normal parturition but are also associated with preterm birth. Studies evaluating such markers in patients with cervical insufficiency have evaluated only their diagnostic potential. Even fewer studies have studied them within the context of cerclage surgery. OBJECTIVES(S): The objective of the study was to evaluate the impact of local and systemic inflammatory markers on the pathogenesis of cervical insufficiency and the effect of cerclage surgery on the local immune microenvironment of women with cervical insufficiency. STUDY DESIGN: We recruited 28 pregnant women (12- 20 weeks' gestation) diagnosed with insufficiency and referred for cerclage surgery and 19 gestational age-matched normal pregnant women as controls. Serum and cervicovaginal fluid samples were collected before and after cerclage surgery and during a routine checkup for normal women and analyzed using a targeted 13-plex proinflammatory cytokine assay. RESULTS: Before surgery, patients with cervical insufficiency had higher levels of interleukin-1b, interleukin-6, interleukin-12, monocyte chemoattractant protein-1 and tumor necrosis factor alpha in cervicovaginal fluid compared to controls, but after surgery, these differences disappeared. No differences were found in serum of insufficiency versus control women. In patients with insufficiency, the levels of interleukin-1b, interleukin-6, interleukin-8, monocyte chemoattractant protein-1, and interferon gamma in cervicovaginal fluid declined significantly after cerclage compared with before intervention, but these changes were not detected in serum. CONCLUSION: Compared with normal women, patients with cervical insufficiency have elevated levels of proinflammatory cytokines in cervicovaginal fluid but not in serum, suggesting a dysregulation of the local immune environment. Cerclage intervention led to a significant decline in these proinflammatory cytokines, suggesting that cerclage may help reduce local inflammation in cervical insufficiency.
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    Cimetidine Reduces Interleukin-6, Matrix Metalloproteinases-1 and-9 Immunoexpression in the Gingival Mucosa of Rat Molars With Induced Periodontal Disease
    (Amer Acad Periodontology, 2017) Oliveira, Priscila Aparecida de [UNIFESP]; Pizzol Junior, Jose Paulo de [UNIFESP]; Longhini, Renata [UNIFESP; Sasso-Cerri, Estela; Cerri, Paulo Sergio
    Background: Histamine seems to act, via H-2 receptor, on inflammatory processes by stimulating interleukin (IL)-6 and matrix metalloproteinase (MMP) release. As cimetidine is an H-2 receptor antagonist, the authors hypothesize that this antiulcer drug reduces IL-6, MMP-1, andMMP-9immunoexpressioningingiva with inducedperiodontal disease (PD). Toconfirmapossible modulatory role of IL-6 on MMPs, the relationship between IL-6/MMP-1 and IL-6/MMP-9 immunoexpression was evaluated. Methods: Forty-six male rats were distributed into the cimetidine group (CimG: received daily intraperitoneal injections of 100 mg/kg of body weight of cimetidine) or the saline group (SG). PD was induced by cotton ligature around the maxillary left first molars (PDSG and PDCimG). The right molars were used as controls (SG and CimG). After 7, 15, 30, and 50 days, maxillary fragments were processed for paraffin embedding or for transmission electron microscopy. Tartrateresistant acid phosphatase (TRAP)-positive osteoclasts in the alveolar process surface and number of IL-6, MMP-1, and MMP-9-immunolabeled cells in the gingival mucosa were quantified. Statistical analyses were performed (P <= 0.05). Results: In PDSG and PDCimG, gingival mucosa exhibited few collagen fibers among numerous inflammatory cells. In PDCimG, the number of TRAP-positive osteoclasts and IL-6, MMP-1, andMMP-9-immunolabeled cells was significantly lower than in PDSG at all periods. A positive correlation between IL-6/MMP-1 and IL-6/MMP-9 was detected in PDSG and PDCimG. Conclusion: Cimetidine decreases bone loss through reduction of osteoclast number and induces reduction of IL-6, MMP-1, and MMP-9 immunoexpression, reinforcing the idea that the beneficial effect of cimetidine in PD may be due to reduction of IL-6 immunolabeling in the inflamed gingival mucosa.
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    Efeito da cimetidina sobre a imunoexpressão das metaloproteinases da matriz -1 e -9 e da interleucina -6 na mucosa gengival com periodontite induzida em molares de ratos
    (Universidade Federal de São Paulo (UNIFESP), 2014-07-30) Oliveira, Priscila Aparecida de [UNIFESP]; Cerri, Estela Sasso Cerri [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Matrix metalloproteinases -1 (MMP-1) and -9 (MMP-9) and interleukin- 6 (IL-6) participate in the loss of support in periodontitis. It has been shown that cimetidine, an antagonist of H2 receptor of histamine, reduces the gingival mucosa inflammation and the alveolar process resorption. In this study, we purposed to verify if the treatment with cimetidine interferes in the of MMP-1, MMP-9 and IL-6 immunoexpression in the gingival mucosa of rat molars with induced periodontitis. Methods: Forty Holtzman rats were divided into 2 groups: sham group and cimetidine group. Periodontitis was induced by ligature in all animals in the 1st upper left molar; the upper right molar was used as control (no ligature). The animals from control group received cimetidine (100mg/kg b.w.) for 7, 15, 30 and 50 days while the animals from the sham group received an equivalent dose of saline solution. After the experimental periods, the animals were euthanized and hemi-maxilla fragments were removed and embedded in paraffin. Some sections were stained with H&E and Picrosirius red; other sections were submitted to immunohistochemical reactions for detection of MMP-1, MMP-9 and IL-6. The numerical density of MMPs and IL-6 immunolabeled cells was obtained and the statistical analysis was perfomed by using ANOVA. The significance level was p?0,05. Results: A significant reduction in the MMP-1, MMP-9 and IL-6 immunolabeling was detected in the gingival mucosa of rats with periodontitis that received cimetidine in comparison to the saline treated group. Statistical differences were not found in the MMP-1, MMP-9 and IL-6 immunoexpression between the animals without ligature that received saline in comparison to those that received only cimeitidine. Conclusion: The results indicate that cimetidine plays a beneficial role in the inflammatory process, including matrix degradation during periodontitis. Regarding the antihistaminergic action of cimetidine, it is possible that this drug interferes, at least in part, in the histamine-dependent processes of inflammation which lead to the periodontal disease.
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    Fish Oil Supplementation Reduces Heart Levels of Interleukin-6 in Rats with Chronic Inflammation due to Epilepsy
    (Frontiers Media Sa, 2017) Nejm, Mariana Bocca [UNIFESP]; Abou Haidar, Andre [UNIFESP]; Hirata, Aparecida Emiko [UNIFESP]; Oyama, Lila Missae [UNIFESP]; de Almeida, Antonio-Carlos Guimaraes; Cysneiros, Roberta Monterazzo; Cavalheiro, Esper Abrao [UNIFESP]; Scorza, Carla Alessandra [UNIFESP]; Scorza, Fulvio Alexandre [UNIFESP]
    Sudden unexpected death in epilepsy (SUDEP) is a major cause of premature death related to epilepsy. The causes of SUDEP remain unknown, but cardiac arrhythmias and asphyxia have been suggested as a major mechanism of this event. Inflammation has been implicated in the pathogenesis of both epilepsy and ventricular arrhythmia, with interleukin-6 (IL-6) being recognized as a crucial orchestrator of inflammatory states. Our group previously reported that levels of IL-6 were increased in the hearts of epileptic rats. In this scenario, anti-inflammatory actions are among the beneficial effects of fish oil dietary supplementation. This investigation revealed that elevated levels of IL-6 in the heart were markedly reduced in epileptic rats that were treated in the long-term with fish oil, suggesting protective anti-inflammatory actions against dangerously high levels of IL-6. Based on these findings, our results suggest beneficial effects of long-term intake of fish oil in reducing the inflammation associated with chronic epilepsy.
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    Interleukin-6-174 G/C promoter gene polymorphism in nasal polyposis and asthma
    (Int Rhinologic Soc, 2013-03-01) Kosugi, Eduardo Macoto [UNIFESP]; De Camargo-Kosugi, Cintia Meirelles [UNIFESP]; Hirai, Elcio Roldan [UNIFESP]; Mendes Neto, José Arruda [UNIFESP]; Gregario, Luis Carlos [UNIFESP]; Cotrim Guerreiro-Da-Silva, Ismael Dale [UNIFESP]; Weckx, Luc Louis Maurice [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background: Interleukin-6 (IL-6) is an inflammatory mediator linked to nasal polyposis and asthma, with a single nucleotide polymorphism - 174 G/C that seems to promote an inflammatory status. We aimed to analyze the relationship between this polymorphism and asthmatic nasal polyposis patients.Methodology: Cross-sectional study to investigate IL-6 - 174 G/C genotypes of 45 nasal polyposis with asthma patients, 63 nasal polyposis-only patients, 45 asthma-only patients and 81 subjects without both diseases. Aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with specific primers followed by restriction enzyme analysis, classifying patients in GG, GC or CC genotype.Results: the GG genotype was the most frequent in all inflammatory groups. Less than 40% of controls presented with the GG genotype. There were significant differences between inflammatory groups and control group. No significant differences were seen when comparing inflammatory groups to each other, other than between nasal polyposis-only group and asthma-only group.Conclusion: the IL-6 74 GG genotype was found more frequently in all inflammatory groups than in controls. This genotype could influence nasal polyposis and asthma, and seems to be more important in the latter.
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    Interleukin-6-174 G/C promoter polymorphism and nasal polyposis
    (Int Rhinologic Soc, 2009-12-01) Kosugi, Eduardo Macoto [UNIFESP]; Camargo-Kosugi, Cintia Meirelles de [UNIFESP]; Maurice Weckx, Luc Louis [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Gregorio, Luiz Carlos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Nasal polyposis is a chronic disease with unknown etiopathogenesis, although inflammatory mechanisms seem to play a role. One of several inflammatory mediators linked to nasal polyposis is Interleukin-6, which has a single. nucleotide polymorphism -174 G/C that seems to promote an inflammatory reaction.Objective: To compare the prevalence of the -174 G/C single nucleotide polymorphism between a group of patients with nasal polyposis and a control group.Method. Cross-sectional study with two groups (thirty two patients with nasal polyposis and fifty five controls) to investigate the -174 G/C polymorphism in blood samples. Asthma, aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with forward primer 5'-ATGCCAAGTGCTGAGTCACTA-3' and reverse primer 5'-GGAAAATCCCACATTTGATA-3', amplifying a 226-bp DNA fragment that contained the 174 position. The amplified fragment can be cleaved by restriction enzyme NlaIII when the -174 position presented the C allele in two fragments of 117 and 109-bp, visualized by electrophoresis, classifying participants in GG, GC and CC.Results: In the nasal polyposis group, 65.62% of the patients had the GG genotype, while in the control group only 41.82% had the GG genotype, a statistically significant difference, with an odds ratio of 2.65.Conclusion: The -174 GG genotype was found more frequency in nasal polyposis patients than in controls, when asthma, aspirin intolerance and atopy were excluded.
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    Serum and cellular interleukin-6 in haemodialysis patients: relationship with energy expenditure
    (Oxford Univ Press, 2007-03-01) Kamimura, Maria Ayako [UNIFESP]; Draibe, Sergio Antonio [UNIFESP]; Dalboni, Maria Aparecida [UNIFESP]; Cendoroglo, Miguel [UNIFESP]; Avesani, Carla M. [UNIFESP]; Manfredi, Silvia R. [UNIFESP]; Canziani, Maria E. F. [UNIFESP]; Cuppari, Lilian [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background. Inflammation is a highly prevalent condition among end-stage renal disease (ESRD) patients and it has been implicated with several metabolic derangements. Considering the harmful effect of hypermetabolism on nutritional status and clinical outcomes of ESRD patients, we aimed to investigate the relationship between proinflammatory cytokine interleukin-6 (IL-6) and energy expenditure in this population.Methods. This cross-sectional study enrolled 80 adult haemodialysis patients for the evaluation of serum IL-6 and energy expenditure. the production of IL-6 by peripheral blood mononuclear cells (PBMCs) (spontaneous and endotoxin-stimulated production) was examined in a subgroup of 30 haemodialysis patients and in 11 healthy control subjects. IL-6 was measured by immunoenzymatic assay. the resting energy expenditure was evaluated by means of indirect calorimetry. Body composition was assessed by bioelectrical impedance analysis and skinfold thicknesses.Results. Serum IL-6 [6.3 (2.2-163.5) pg/ml] correlated positively with age (R = 0.26; P = 0.02) and C-reactive protein (R = 0.31; P < 0.01). Resting energy expenditure correlated positively with lean body mass (R = 0.68; P < 0.001) and BMI (R = 0.44; P < 0.001), and negatively with Kt/V (R = -0.37; P < 0.01). in the multivariate analysis, controlling for age and lean body mass, serum IL-6 was positively associated with resting energy expenditure (n = 80; beta = 2.4; P = 0.01). the production of IL-6 by PBMCs did not reach statistically significant differences between patients and controls [spontaneous production 6541 (96-7739) pg/ml vs 3410 (50-7806) pg/ml, respectively; and stimulated production 6530 (579-7671) pg/ml vs 5304 (1527-7670) pg/ml, respectively]. IL-6 secreted by monocytes showed no association with either serum IL-6 or resting energy expenditure.Conclusion. Serum IL-6 was associated with an increase of energy expenditure in haemodialysis patients.