Navegando por Palavras-chave "liver regeneration"

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    Efeito da infusão de células-tronco mesenquimais na regeneração do fígado após hepatectomia a 70%: modelo experimental em ratos
    (Universidade Federal de São Paulo (UNIFESP), 2015-12-18) Alves, Ana Karina Soares [UNIFESP]; Linhares, Marcelo Moura Linhares [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Major hepatic resections still have high levels of morbimortality, mainly due the post-operatory hepatic failure. In order to enable fast regeneration of the remnant liver techniques as previous embolization or portal vein ligature, two-stage hepatectomy and the ALPPS have been used, yet the insufficient hepatic mass continues to be a challenge. The cell therapy has been speculated as a promising therapeutic arsenal to accelerate or improve the hepatic regeneration after extended resections. The aim of this study is to evaluate the effect of the mesenchymal stem cells on the hepatic regeneration in rats submitted under 70% hepatectomy. Methods: in fourty Winstar rats submitted under 70% hepatectomy it was infused 106 of mesenchymal stem cells (trial group) or saline solution (control group) through portal vein. These groups were divided in early and late subgroups (euthanasia on the third and fifth post operative day, respectively). Outcomes of the hepatic regeneration tax, albumin variation, TGO, TGP and FA, cell proliferation index (ki-67) and the number of mitosis were compared. Results: Significant outcomes in the hepatic regeneration tax, number of mitosis and cell proliferation index were not observed. Likewise there was no difference of albumin levels variation, TGO and FA. It was found significant variation in the levels of TGP, higher in the trial group. Conclusions: It was not observed improvement of the hepatic regeneration tax on the third and fifth post hepatectomy day among rats that received cellular therapy. There was also no improvement of hepatic function, of cell proliferation index and of the number of mitosis in the groups that were submitted under the mesenchymal stem cells infusion.
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    Plasma-kallikrein clearance during liver regeneration after partial hepatectomy in the rat
    (Munksgaard Int Publ Ltd, 1998-12-01) Braulio, Valeria Bender; Kouyoumdjian, Maria [UNIFESP]; Zucoloto, Sergio; Figueiredo, Florencio; Borges, Durval Rosa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Rio de Janeiro (UFRJ); Universidade de São Paulo (USP)
    Aims/Background. The liver clears circulating plasma-kallikrein through a receptor-mediated endocytosis process: an initial fast phase is followed by a slow exponential phase. Methods: To determine whether the clearance rate of plasma-kallikrein is affected during liver regeneration, we perfused isolated rat livers with rat plasma-kallikrein (rPK) at 0, 1, 2, 3 and 7 days after partial hepatectomy or sham operation. Results. Liver regeneration was followed by the expression of the proliferating-cell nuclear antigen (PCNA) labeling index. The serum concentration of alpha(2)-macroglobulin, an acute phase protein in rats, was measured. At day 1, the fast phase of rPK clearance rate increased in hepatectomized rats when compared with day 0 (4.9+/-0.4 and 3.7+/-0.4 mU/g liver min, p<0.05). However, at day 2, the rPK fast phase clearance rate dropped significantly (2.6+/-0.2, p<0.05), when compared with day 1. No difference was found among the sham groups at different days of hepatectomy. These changes seem to be independent of the acute phase reaction. The regenerative liver weight increased continuously during the observation period. PCNA expression increased significantly after hepatectomy, with maximal PCNA-labeling indices at days 1 and 2, declining thereafter. Conclusion. The rPK fast phase clearance rate changes during liver regeneration, with a zenith occurring when PCNA labeling index is maximal (day 1) and a nadir occurring at the mitotic phase (day 2).