Navegando por Palavras-chave "metaloproteinases da matriz"

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    Efeito da cimetidina sobre a imunoexpressão das metaloproteinases da matriz -1 e -9 e da interleucina -6 na mucosa gengival com periodontite induzida em molares de ratos
    (Universidade Federal de São Paulo (UNIFESP), 2014-07-30) Oliveira, Priscila Aparecida de [UNIFESP]; Cerri, Estela Sasso Cerri [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Matrix metalloproteinases -1 (MMP-1) and -9 (MMP-9) and interleukin- 6 (IL-6) participate in the loss of support in periodontitis. It has been shown that cimetidine, an antagonist of H2 receptor of histamine, reduces the gingival mucosa inflammation and the alveolar process resorption. In this study, we purposed to verify if the treatment with cimetidine interferes in the of MMP-1, MMP-9 and IL-6 immunoexpression in the gingival mucosa of rat molars with induced periodontitis. Methods: Forty Holtzman rats were divided into 2 groups: sham group and cimetidine group. Periodontitis was induced by ligature in all animals in the 1st upper left molar; the upper right molar was used as control (no ligature). The animals from control group received cimetidine (100mg/kg b.w.) for 7, 15, 30 and 50 days while the animals from the sham group received an equivalent dose of saline solution. After the experimental periods, the animals were euthanized and hemi-maxilla fragments were removed and embedded in paraffin. Some sections were stained with H&E and Picrosirius red; other sections were submitted to immunohistochemical reactions for detection of MMP-1, MMP-9 and IL-6. The numerical density of MMPs and IL-6 immunolabeled cells was obtained and the statistical analysis was perfomed by using ANOVA. The significance level was p?0,05. Results: A significant reduction in the MMP-1, MMP-9 and IL-6 immunolabeling was detected in the gingival mucosa of rats with periodontitis that received cimetidine in comparison to the saline treated group. Statistical differences were not found in the MMP-1, MMP-9 and IL-6 immunoexpression between the animals without ligature that received saline in comparison to those that received only cimeitidine. Conclusion: The results indicate that cimetidine plays a beneficial role in the inflammatory process, including matrix degradation during periodontitis. Regarding the antihistaminergic action of cimetidine, it is possible that this drug interferes, at least in part, in the histamine-dependent processes of inflammation which lead to the periodontal disease.