Navegando por Palavras-chave "progesterone receptor"
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- ItemSomente MetadadadosEstrogen and progesterone receptors in gastric and colorectal cancer(H G E Update Medical Publ Ltd., 1999-11-01) Oshima, Celina Tizuko Fujiyama [UNIFESP]; Wonraht, D. R.; Catarino, Regina Maria [UNIFESP]; Mattos, D.; Forones, Nora Manoukian [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)BACKGROUND/AIMS: Estrogen (ER) and progesterone receptors (PR) have been evaluated in gastrointestinal cancer by several groups with conflicting results. The aim of the study is to examine the presence of these receptors in gastric and colon cancer.METHODOLOGY: Estrogen (ER) and progesterone (PR) receptors were assayed by the dextran-coated charcoal adsorption method from malignant and normal adjacent tissues in 16 patients with gastric adenocarcinomas and in 10 with colorectal adenocarcinomas.RESULTS: In gastric cancer, ER were detected in 62.5% and PR in 75% of the patients. In colorectal cancer, the ER and PR were detected in 60% of the patients. The binding activity ranged from 1.14-9.27 fmol/mg protein for estradiol and from 1.43-10.84 fmol/mg protein for progesterone. ER and PR were detected in normal gastric tissue in 62.5% and in 50%, respectively. In the normal colorectal tissue the ER and PR were detected in 30% and 50%. ER ranged from 1.20-16.63,fmol/mg protein for estradiol and from 1.44-9.94 fmol/mg protein for progesterone. There was no statistical difference in levels of ER and PR in both tissues.CONCLUSIONS: ER and PR were detected in normal and cancer tissues in low levels, suggesting a feature of the tissue rather than a consequence of a malignant process. Eventual role of ER or PR in these cancers remains to be elucidated.
- ItemSomente MetadadadosGenetic polymorphisms of cytochrome P450c17 alpha (CYP17) and progesterone receptor genes (PROGINS) in the assessment of endometriosis risk(Parthenon Publishing Group, 2007-01-01) Carvalho, Cristina Valletta de [UNIFESP]; Nogueira-de-Souza, Naiara Corrêa [UNIFESP]; Costa, Ana Maria Massad [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Girão, Manoel João Batista [UNIFESP]; D'Amora, Paulo [UNIFESP]; Schor, Eduardo [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)We designed the present study in order to evaluate the eventual role of polymorphisms in the genes encoding cytochrome P450c17 alpha (CYP17) and the progesterone receptor (PROGINS) as risk factors for endometriosis development. Eligible cases consisted of 121 women with surgically confirmed endometriosis who underwent treatment in a hospital in São Paulo, Brazil during the period from September 2003 to September 2005. the 281 controls were participants with normal gynecological as well as pelvic ultrasound evaluation, who did not have any gynecological conditions during their reproductive lives such as pelvic pain and/or dyspareunia nor infertility history. Genomic DNA was obtained from buccal cells and processed for DNA extraction using the GFX DNA extraction kit (GE Healthcare). the CYP17 (- 34T-->C) polymerase chain reaction-restriction fragment length polymorphism assay has been described previously, as has the progesterone receptor polymorphism (PROGINS) detection assay. PROGINS heterozygosis genotype frequencies were shown to be statistically higher in endometriosis cases compared with controls. On the other hand, differences in the CYP17 polymorphism (- 34T --> C) frequencies were not even close to significance (p = 0.278) according to our findings.
- ItemSomente MetadadadosThe progesterone receptor gene polymorphism, PROGINS, may be a factor related to the development of uterine fibroids(Elsevier B.V., 2007-05-01) Gomes, Mariano Tamura Vieira [UNIFESP]; Castro, Rodrigo de Aquino [UNIFESP]; Villanova, Fabiola Elizabeth [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Lima, Geraldo Rodrigues de [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To assess the possible association between the polymorphic allele of the progesterone receptor gene, named PROGINS, and uterine leiomyomas.Design: Case-control study.Setting: Department of Gynecology. Teaching hospital.Patient(s): One hundred twenty-two premenopausal women with fibroids and 125 postmenopausal controls not presenting the disease.Intervention(s): the subjects were classified as White or non-White (Black and Mulatto) and the progesterone receptor genotyping was performed, with DNA extracted from uterus in cases and from peripheric blood in controls and submitted to polymerase chain reaction (PCR) and agarose gel electrophoresis.Main Outcome Measure(s): the presence of the PROGINS allele was recorded, and its frequency as well as the genotypic distribution among cases and controls were compared according to race.Result(s): PROGINS-positive genotypes (heterozygous or mutant homozygous) were found in 19% of White and 11% of non-White women, and allelic frequency of PROGINS in the groups was 10.4% and 6.2%, respectively. Comparing patients and controls, we observed a significant difference among non-White women, both regarding presence of PROGINS-positive genotypes (4.9% vs. 25%, respectively), and PROGINS allele frequency (3.3% vs. 12.5%, respectively). There was no significant difference in PROGINS-positive genotypes among White cases and controls (16.4% vs. 20.6%, respectively), and in their allelic frequency (8.2% vs. 11.9%, respectively). the odds ratio showed reduced risk of fibroids related to PROGINS-positive genotypes in non-White women (odds ratio = 0.16, 95% confidence interval: 0.04-0.66), but not among White subjects (odds ratio = 0.76, 95% confidence interval: 0.33-1.74).Conclusion(s): the PROGINS polymorphism revealed to be protective in terms of uterine fibroids in Brazilian non-White women.