Navegando por Palavras-chave "shared epitope"

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    Estudo da frequência dos alelos de HLA-DRB1 em pacientes brasileiros com artrite reumatoide
    (Sociedade Brasileira de Reumatologia, 2011-10-01) Usnayo, Magali Justina Gómez; Andrade, Luiz Eduardo Coelho [UNIFESP]; Alarcon, Renata Trigueirinho [UNIFESP]; Oliveira, Juliana Cardoso; Silva, Gustavo Milson Fabrício; Bendet, Izidro; Burlingame, Rufus; Porto, Luis Cristóvão; Pinheiro, Geraldo da Rocha Castelar; Universidade Estadual do Rio de Janeiro Programa de Pós-graduação em Ciências Médicas; Universidade Federal de São Paulo (UNIFESP); UERJ Laboratório de Histocompatibilidade e Criopreservação Programa de Pós-graduação em Biologia Humana e Experimental; DASA Sérgio Franco Medicina Diagnóstica; INOVA Diagnostics, Inc; UERJ Departamento de Histologia e Embriologia Laboratório de Histocompatibilidade e Criopreservação; UERJ Faculdade de Ciências Médicas
    The HLA-DRB1 alleles encoding an amino acid sequence (QKRAA/QRRAA/RRRAA) at position 70 74 of the third hypervariable region of the β1 chain of the HLA-DRB1 gene, called shared epitope (SE), are associated with increased susceptibility to and severity of rheumatoid arthritis (RA) in different populations. OBJECTIVE: To determine the frequency of HLA-DRB1 alleles in Brazilian patients with RA and their association with rheumatoid factor (RF) and anti-citrullinated peptide antibodies (ACPA). METHODS: Four hundred and twelve patients with RA (ACR 1987) and 215 controls were included. HLA-DRB1 typing was performed by use of polymerase chain reaction (PCR) with specific primers and hybridization with sequence-specific oligonucleotide probe (SSOP). ACPA was measured by use of the ELISA technique and RF by nephelometry. The statistical analysis comprised the chi-square and Student t tests and logistic regression. RESULTS: HLA-DRB1*04:01, *04:04, *04:05 alleles were associated with RA (P < 0.05); despite the wide confidence interval, it is worth noting the association between the DRB1*09:01 allele and RA (P < 0.05). HLA-DRB1 SE+ alleles were observed in 62.8% of the patients and in 31.1% of controls (OR 3.62; P < 0.001) and were associated with ACPA (OR 2.03; P < 0.001). DRB1-DERAA alleles showed a protective effect against RA (OR 0.42; P < 0.001). CONCLUSION: In a sample of Brazilian patients with RA, most of whom of mixed heritage, HLA-DRB1 SE+ alleles were associated with susceptibility to disease and presence of ACPA.