Navegando por Palavras-chave "shrimp"

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    Cockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergens
    (Mosby-year Book Inc, 1999-08-01) Santos, ABR; Chapman, M. D.; Aalberse, R. C.; Vailes, L. D.; Ferriani, VPL; Oliver, C.; Rizzo, M. C.; Naspitz, C. K.; Arruda, L. K.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Netherlands Red Cross; Univ Virginia
    Background: Cockroaches produce several proteins that induce IgE antibody responses. Although cockroaches are abundant in warm and humid areas, sensitization to cockroach allergens has not been investigated in Brazil.Objective: the aims of this study were to investigate the frequency of cockroach allergy among patients with asthma, rhinitis, or both in Brazil and to identify American cockroach allergens.Methods: Skin tests using cockroach extracts were performed on children and young adults with asthma, rhinitis, or both. A Periplaneta americana complementary (c)DNA library was screened by using IgE antibodies from Brazilian patients allergic to cockroaches. Reactivity of an mAb directed to Dermatophagoides pteronyssinus tropomyosin against cockroach tissue was examined by immunofluorescence.Results: Cockroach allergy was present in 55% and 79% of the patients, as determined by using skin prick tests alone or combined prick and intradermal tests, respectively. Five cDNA clones reacted with IgE antibody and contained the same sequence. A representative clone (1300 bp), pa12, coded for a protein that reacted with 50% of the sera from patients allergic to cockroaches on plaque immunoassay and showed a high degree of homology to tropomyosins, particularly those from invertebrates. P americana tropomyosin showed 80%, 81%, and 82% sequence identity to tropomyosins from D pteronyssinus, D farinae, and shrimp, respectively, which have been previously defined as important allergens. An mAb directed against D pteronyssinus tropomyosin, which also recognizes shrimp tropomyosin, showed binding to cockroach striated muscle.Conclusion: Our results support the recommendation that cockroach extracts should be routinely used for the evaluation of patients with asthma, rhinitis, or both in Brazil. the identification of P americana tropomyosin as an important allergen Hill make it possible to investigate cross-reactivity among cockroaches, mites, and food derived from invertebrates.
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    Format and mode of artificial substrate fixation affect the performance of Litopenaeus vannamei in high-density rearing systems
    (Wiley, 2018) Schveitzer, Rodrigo [UNIFESP]; Zanetti Leite, Thais Sabino [UNIFESP]; Orteney, Natacha Ester [UNIFESP]; Temistocles Menezes, Fabrini Copetti [UNIFESP]; Medeiros, Igor Dias [UNIFESP]
    We investigated whether the positive impacts of artificial substrates on shrimp performance are altered in any way by their format or mode of fixation in the tanks. To examine this question, substrates were fixed vertically in the water column in three different configurations: SCF treatment (Substrate Completely Fixed), SPF treatment (Substrate Partially Fixed) and SFF (Substrate in Frond Format). Another treatment received no substrate and served as control (WS=Without Substrate). The shrimp were cultured for 38days in intensive biofloc culture tanks at a stocking density of 1,125 shrimp m(-3). In general, water quality variables were similar among treatments and remained within the appropriate range for shrimp culture. The final biomass was higher (8.5kg m(-3)) and the feed conversion ratio (FCR) lower (1.6) in all tanks with substrates when compared with the WS treatment tanks (final biomass=6.3kg m(-3) and FCR=3.1). However, only shrimp from the SCF and SPF treatments had a higher survival rate (>95.0%) compared to those in WS tanks (75.9%), which was statistically similar to the SFF treatment (88.0%). These results show that substrate format and its mode of fixation in tanks can alter shrimp performance. In well-aerated intensive tanks, substrates in frond format are constantly pushed to the tank surface, making it difficult for shrimp to adhere to the screens. In such situation, the extra surface provided by the substrates is not always available to the shrimp, a fact that minimizes the positive effects of substrate.