Navegando por Palavras-chave "steroid hormones"

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    A influência do índice de massa corporal na fisiologia do folículo ovariano em ciclos de reprodução assistida
    (Universidade Federal de São Paulo (UNIFESP), 2013-04-03) Porciuncula, Patricia Marafon [UNIFESP]; Bertolla, Ricardo Pimenta Bertolla [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To observe the influence of Body Mass Index (BMI) on the follicular metabolism of women submitted to controlled ovarian hyperstimulation. Methods: A prospective study was carried out including 129 follicular fluid samples from women submitted to controlled ovarian hyperstimulation and classified, according to BMI, in three groups according to the World Health Organization: eutrophic (20 to <25kg/m2), overweight (25 to <30kg/m2), and obese (>30kg/m2). Follicular fluid samples were obtained by ultrasound-guided transvaginal ovarian aspiration following controlled ovarian hyperstimulation. Immediately after collection, the follicular fluid samples were centrifuged to remove cellular debris and frozen until analysis. For quantification of the steroid hormones estradiol, progesterone, testosterone, androstenedione, and cortisol, tandem mass spectrometry was utilized (ID-LC-MS/MS). For proteomic analysis, three pools were formed (one for each group, normalized to total protein in each sample), and proteins were quantified and identified utilizing the shotgun proteomics approach nanoUPLC-nanoESI-MSE, resulting in ?Identified? and ?Quantified and Identified? proteins. Proteins observed in only one replicate were excluded from the study. For steroid profiling, groups were compared using one-way ANOVA followed by a Least Significant Differences posthoc test, or using a Kruskal-Wallis followed by a Mann-Whitney test. For the proteomics experiment a Kruskal-Wallis test was used, followed by a Mann-Whitney test. An alpha of 5% was adopted in the study. Protein lists were utilized for functional enrichment analysis. Results: Regarding the steroid profile, differences were observed between the BMI groups. The obesity group presented lower progesterone levels and higher androstenedione levels when compared to the other two groups. Also, estradiol, testosterone, and cortisol were higher in both overweight and obese groups when compared to eutrophic controls. Regarding proteomics, 284 proteins were identified, of which 152 were quantified. Major enriched functions were related to immune response, inflammation and coagulation. Moreover, seven proteins were hyperexpressed only in the obese patients. Finally, 20 proteins were exclusively expressed in the obesity group, 10 in the overweight group, and 19 in the eutrophic group. Conclusion: Follicular fluid metabolism is altered due to obesity in patients submitted to controlled ovarian hyperstimulation. The follicular fluid of overweight and obese patients presents altered steroid profiles, with increased estradiol, testosterone, and cortisol, and obese patients present increased androstenedione and decreased progesterone. Also, overweightness and obesity alter the follicular fluid protein profile of patients submitted to controlled ovarian hyperstimulation, with enriched immune and inflammatory response functions.