Navegando por Palavras-chave "tissue microarray"
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- ItemSomente MetadadadosClaudin-7 down-regulation is an important feature in oral squamous cell carcinoma(Wiley-Blackwell, 2010-11-01) Lourenco, Silvia Vanessa; Coutinho-Camillo, Claudia Malheiros; Cavicchioli Buim, Marcilei Elisa; Carvalho, Ana Carolina de [UNIFESP]; Lessa, Roberta Cardim; Pereira, Claudia Maria; Vettore, Andre Luiz [UNIFESP]; Carvalho, Andre Lopes; Fregnani, Jose Humberto; Kowalski, Luiz Paulo; Soares, Fernando Augusto; Universidade de São Paulo (USP); Hosp AC Camargo Fund Antonio Prudente; Universidade Federal de São Paulo (UNIFESP); Ludwig Inst Canc Res; Hosp Canc BarretosAims:Claudins, a large family of essential tight junction (TJ) proteins, are abnormally regulated in human carcinomas, especially claudin-7. the aim of this study was to investigate claudin-7 expression and alterations in oral squamous cell carcinoma (OSCC).Methods and results:Expression of claudin-7 was analysed in 132 cases of OSCC organized in a tissue microarray. Claudin-7 mRNA transcript was evaluated using real-time polymerase chain reaction and the methylation status of the promoter was also assessed. Claudin-7 was negative in 58.3% of the cases. Loss of claudin-7 protein expression was associated with recurrence (P = 0.019), tumour size (P = 0.014), clinical stage of OSCC (P = 0.055) and disease-free survival (P = 0.015). Down-regulation of the claudin-7 mRNA transcripts was observed in 78% of the cases, in accordance with immunoexpression. Analysis of the methylation status of the promoter region of claudin-7 revealed that treatment of O28 cells (that did not express claudin-7 mRNA transcripts) with 5-Aza-2'-Deoxycytidine (5-Aza-dC) led to the re-expression of claudin-7 mRNA transcript.Conclusion:Loss of claudin-7 expression is associated with important subcellular processes in OSCC with impact on clinical parameters.
- ItemSomente MetadadadosEndometrial leukemia inhibitory factor as a predictor of pregnancy after in vitro fertilization(Elsevier B.V., 2008-07-01) Serafini, Paulo; Rocha, Andre M.; Osorio, Cyntia T.; Silva, Ismael da [UNIFESP]; Motta, Eduardo L. [UNIFESP]; Baracat, Edmund C.; Huntington Reproduct Ctr; Universidade de São Paulo (USP); Hosp Canc AC Camargo; Universidade Federal de São Paulo (UNIFESP)Objective: To determine whether there is an association between endometrial expression of leukemia inhibitory factor (LIF) in the luteal phase of the menstrual cycle preceding in vitro fertilization (IVF) and treatment outcome. Methods: Biopsy specimens from the endometria of 52 women in the luteal. phase were immunostained against LIF Embryo culture and transfer were done according to standard procedures. Results: Clinical pregnancy occurred in 39% of the women following IVF, and strong endometrial immunohistochemical staining for LIF was associated with pregnancy (P=0.01). the women with a strong LIF expression had a 6.4-fold higher chance of becoming pregnant than those with weaker intensities (P=0.005). Conclusion: Endometrial expression of LIF during the luteal phase can be used as a predictor of IVF success. (C) 2008 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
- ItemSomente MetadadadosExpression Profiling of Cell Cycle Regulatory Proteins in Oropharyngeal Carcinomas Using Tissue Microarrays(Int Inst Anticancer Research, 2010-05-01) Ribeiro, Daniel Araki [UNIFESP]; Nascimento, Fábio Dupart [UNIFESP]; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Gomes, Thiago Simao [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Aim: The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis. Patients and Methods: Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays. Results: Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx. Conclusion: Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.
- ItemSomente MetadadadosHepatoblastomas and liver development: A study of cytokeratin immunoexpression in twenty-nine hepatoblastomas(Alliance Communications Group Division Allen Press, 2006-05-01) Cajaiba, Mariana Morais; Neves, Jose Ivanildo; Casarotti, Fabiana Facco; De Camargo, Beatriz; Chapchap, Paulo; Sredni, Simone Treiger; Soares, Fernando Augusto; Hosp Canc AC Camargo São Paulo Brazil; Universidade Federal de São Paulo (UNIFESP)Hepatoblastomas (HBs) recapitulate liver development. It is possible that HBs result from malignant transformation of hepatic precursor cells, and they may reflect a blockage in normal development. Here we study the expression of cytokeratins (CKs) in order to delineate the immunoprofile and relationship with liver development, as well as vimentin and alphafetoprotein (AFP), of HBs. Immunohistochemistry was performed in a tissue microarray (TMA) containing representative areas of 18 HBs (fetal and/or embryonal and/or mesenchymal); we also reviewed I I cases not included in the TMA. No cases stained for CKs 1, 5/6, 7, 10, 13, 15, 16, 20, and 34 beta E12. CK8 stained 73.07% of fetal, 50% of embryonal, and 18% of mesenchymal areas. CK18 stained 100% of epithelial areas. CK19 staining was intense and diffuse in 100% of embryonal samples, but it was weaker in fetal areas (66.66%). AE1 stained epithelial areas in all cases, and it stained 29.41% of mesenchymal areas. AE3 stained 84.61% of embryonal and 60% of fetal components. AE1/AE3 showed stronger staining in embryonal (100%) than in fetal areas (76.92%). Vimentin staining was strong in embryonal (66.66%) and mesenchymal (84.61%) components but weak in fetal areas (8%). Alphafetoprotein was positive in only 20% of fetal and 70% of embryonal areas. Our results support the hypothesis that immunoexpression of HBs follows the stages of normal liver development. Embryonal areas look less differentiated, expressing vimentin and biliary epithelium CKs, whereas fetal areas display a
- ItemSomente MetadadadosProtein profile of the luteal phase endometrium by tissue microarray assessment(Informa Healthcare, 2009-01-01) Serafini, Paulo; Rocha, Andre Monteiro da; Osório, Cintia Aparecida Bueno [UNIFESP]; Smith, Gary Daniel; Hassun Filho, Pericles Assad [UNIFESP]; Silva, Ismael Guerreiro Dale Cotrim Guerreiro da [UNIFESP]; Motta, Eduardo Leme Alves da [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Huntington Reprod Med; Universidade de São Paulo (USP); Hosp Canc AC Camargo; Univ Michigan; Genesis Genet Brazil; Universidade Federal de São Paulo (UNIFESP)To investigate the luteal phase endometrial expression of leukemia inhibitor factor (LIF), insulin-like growth factor 1 (IGF-1), progesterone receptor (PR), claudin 4 (CLDN4), vascular-endothelial growth factor receptor 3 (VEGFR-3), bone morphogenetic protein 4 (BMP-4) and citokeratin 7 (CK-7), we obtained luteal phase endometrial samples from 52 women. Samples were dated and integrated using a tissue microarray (TMA). Samples were immunostained for LIF, IGF-1, PR, CLDN4, VEGFR-3, BMP-4 and CK-7. Frequencies of positive expressions at the early, mid and late luteal phases were compared by two proportions test. Concomitant expression of these proteins was assessed with Chi-square or Fischer's test. the frequency of LIF was positively correlated to the frequency of IGF-1 (r = 0.99; p < 0.05) and PR (r = 0.99; p < 0.05), and the correlation between IGF-1 and PR tended to be significant (r = 0.98; p < 0.1). the expression of PR was associated with the absence of CLDN4 (p < 0.001). Thus, expression of LIF, IGF-1 and PR are correlated during the luteal phase, and immunohistochemistry for these proteins might be used to assist in the assessment of endometrial maturation. in addition, the expression of CLDN4 and PR was not concomitant, warranting further investigation on the relationship of their endometrial expression.
- ItemSomente MetadadadosThe Role of Metalloendopeptidases in Oropharyngeal Carcinomas Assessed by Tissue Microarray(Int Inst Anticancer Research, 2011-11-01) Ribeiro, Daniel Araki [UNIFESP]; Nascimento, Fábio Dupart [UNIFESP]; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Noguti, Juliana [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ihara, Silvia Saiuli Miki [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The goal of this study was to investigate the expression of some metalloendopeptidases in squamous cell carcinomas of the oropharynx as well as its relation to histological differentiation, staging of disease, and prognosis. Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of EP24.15 and EP24.16 by means of tissue microarrays. Expression of EP24.15 or EP24.16 was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinomas of the oropharynx. In summary, our results support the notion that EP24.15 and EP24.16 are expressed in carcinoma of the oropharynx; however, these do not appear to be suitable biomarkers for histological grading, disease stage or recurrence as depicted by tissue microarrays and immunohistochemistry.