Navegando por Palavras-chave "uterine leiomyoma"

Agora exibindo 1 - 4 de 4
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Association of the CYP17 gene polymorphism with risk for uterine leiomyoma in Brazilian women
    (Informa Healthcare, 2008-01-01) Vieira, Lucinda Coelho Esperança [UNIFESP]; Gomes, Mariano Tamura Vieira [UNIFESP]; Castro, Rodrigo de Aquino [UNIFESP]; Nogueira-de-Souza, Naiara Corrêa [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background. Uterine leiomyoma is the most common pelvic tumor in women of reproductive age. It is well established that endogenous sex hormones are involved in disease pathogenesis, and polymorphisms in genes encoding enzymes which act in the metabolism of steroid hormones, such as that for cytochrome P450c17 enzyme (CYP17), may therefore play a role in fibroid genesis. Variations in this gene have been thought to influence the susceptibility to hormone-related diseases. A single nucleotide polymorphism (T -> C) [rs1042386] in promoter region of CYP17 may alter its transcription. the present study was conducted to investigate the association between this polymorphism and the presence of uterine leiomyoma in Brazilian women.Methods. Genotyping of CYP17 was performed in 121 uterine fibroid patients and 120 unaffected women, using polymerase chain reaction and restriction fragment-length polymorphism analysis.Results. No significant difference in the CYP17 genotype distribution was noted between cases and controls (p=0.165). Conclusion. These findings suggest that the CYP17 gene polymorphism studied is unlikely to be associated with risk for uterine leiomyoma in Brazilian women.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Estrogen receptor alpha polymorphism and susceptibility to uterine leiomyoma
    (Elsevier B.V., 2006-11-01) Villanova, Fabiola E.; Andrade, Priscila M.; Otsuka, Audrey Y.; Gomes, Mariano T. V.; Leal, Elcio S.; Castro, Rodrigo A.; Girao, Manoel J. B. C.; Nishimura, Eddy; Baracat, Edmund C.; Silva, Ismael D. C. G.; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Perola Byington Hosp
    Uterine leiomyoma is the most frequent pelvic tumor found in female genital tract. Some studies have suggested an association between single nucleotide polymorphisms (SNPs) in estrogen receptors genes with susceptibility in developing uterine leiomyoma. in this work, we estimated the frequency of two SNPs: one located in the intron 1 (rs9322331) and other in the exon 1 (rs17847075) of the estrogen receptor alpha (ESR1) gene in 125 women with uterine leiomyoma and 125 healthy women. To do this we used a PCR-RFLP method with Mspl and HaeIII restriction enzymes to respectively detect C/T SNPs in the intron 1 and in the exon 1 of ESR1. To our knowledge this is the first study aimed to investigate the association of ESR1 SNPs with the risk of developing uterine leiomyoma in Brazilian women. Our results showed that the allele frequencies of the exon 1 and the intron 1 of the ESR1 gene did not differ between cases and controls (P=0.325 and 0.175, respectively). Furthermore, our findings provided little support for the association of these SNPs on ESR1 with leiomyoma. However, we found that the SNP in the intron 1 of the ESR1 gene was underrepresented in the Brazilian female population.(C) 2006 Elsevier Inc. All rights reserved.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    The progesterone receptor gene polymorphism, PROGINS, may be a factor related to the development of uterine fibroids
    (Elsevier B.V., 2007-05-01) Gomes, Mariano Tamura Vieira [UNIFESP]; Castro, Rodrigo de Aquino [UNIFESP]; Villanova, Fabiola Elizabeth [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Lima, Geraldo Rodrigues de [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To assess the possible association between the polymorphic allele of the progesterone receptor gene, named PROGINS, and uterine leiomyomas.Design: Case-control study.Setting: Department of Gynecology. Teaching hospital.Patient(s): One hundred twenty-two premenopausal women with fibroids and 125 postmenopausal controls not presenting the disease.Intervention(s): the subjects were classified as White or non-White (Black and Mulatto) and the progesterone receptor genotyping was performed, with DNA extracted from uterus in cases and from peripheric blood in controls and submitted to polymerase chain reaction (PCR) and agarose gel electrophoresis.Main Outcome Measure(s): the presence of the PROGINS allele was recorded, and its frequency as well as the genotypic distribution among cases and controls were compared according to race.Result(s): PROGINS-positive genotypes (heterozygous or mutant homozygous) were found in 19% of White and 11% of non-White women, and allelic frequency of PROGINS in the groups was 10.4% and 6.2%, respectively. Comparing patients and controls, we observed a significant difference among non-White women, both regarding presence of PROGINS-positive genotypes (4.9% vs. 25%, respectively), and PROGINS allele frequency (3.3% vs. 12.5%, respectively). There was no significant difference in PROGINS-positive genotypes among White cases and controls (16.4% vs. 20.6%, respectively), and in their allelic frequency (8.2% vs. 11.9%, respectively). the odds ratio showed reduced risk of fibroids related to PROGINS-positive genotypes in non-White women (odds ratio = 0.16, 95% confidence interval: 0.04-0.66), but not among White subjects (odds ratio = 0.76, 95% confidence interval: 0.33-1.74).Conclusion(s): the PROGINS polymorphism revealed to be protective in terms of uterine fibroids in Brazilian non-White women.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Uterine volume and menstrual patterns in users of the levonorgestrel-releasing intrauterine system with idiopathic menorrhagia or menorrhagia due to leiomyomas
    (Elsevier B.V., 2007-03-01) Magalhaes, Jarbas; Aldrighi, Jose Mendes; Lima, Geraldo Rodrigues de; Personna Ctr Gynecol & Womans Hlth; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Background: A prospective cohort study was carried out to evaluate uterine volume and the volume of uterine leiomyomas in women using the levonorgestrel intrauterine system (LNG-IUS) to treat idiopathic menorrhagia (n=32) and menorrhagia, due to leiomyomas (n=27). A control group used the device as a contraceptive method (n = 2 8).Methods: Clinical and ultrasonographic evaluations were carried out at insertion and at 3, 6, 12, 24 and 36 months later. Total uterine volume and the volume of the leiomyomas were calculated using the ellipsoid formula (anteroposterior diameter) x (transverse diameter)x(longitudinal diameter) x (4/3) x (pi). in the case of multiple leiomyomas, the volume of each myoma was added to calculate the total volume of leiomyomas in each patient. Menstrual bleeding episodes were recorded.Results: Uterine volume decreased significantly in both groups of menorrhagic patients but not in the control group. in the group of women with idiopathic menorrhagia, a mean reduction of 36.4 +/- 15.3 (S.D.) cm(3) (from 127.1 cm(3) to 90.7 cm(3)) was observed (p=.041), and a greater and more significant mean reduction of 63.6 +/- 19.0 (S.D.) cm(3) (from 156.6 cm(3) to 93 CM) occurred in the group of women with leiomyomas (p=.014). in the contraception group, the reduction was of only 2.9 +/- 5.4 (S.D.) cm(3) in mean uterine volume (from 70.3 cm 3 to 67.4 cm 3), which was not statistically significant (p=.085). the mean volume of leiomyomas decreased by 5.2 +/- 3.1 (S.D.) cm 3 (from 12.8 cm(3) to 7.6 cm(3)) after 3 years of use, but this difference was not significant (p =.4099). After 3 6 months of use, amenorrhea and oligomenorrhea were the most frequent bleeding patterns, occurring in 45-57% and 33-39% of users in the three groups, respectively. Amenorrhea was higher in the contraception group (57.1%) and in women with idiopathic menorrhagia (53.4%) than women in the group with menorrhagia due to leiomyomas (44.5%) (p=.027). Moreover, the prevalence of spotting was almost three times higher (11%) in women with menorrhagia caused by leiomyomas and nearly double (7.7%) in the idiopathic menorrhagia group when compared with 4% in the control contraception group (p=.024).Conclusion: the LNG-IUS significantly reduces uterine volume in women with menorrhagia with and without leiomyoma; however, it does not significantly reduce the volume of leiomyomas. (c) 2007 Elsevier Inc. All rights reserved.