Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis?

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dc.contributor.authorSouza, A. W. S. de [UNIFESP]
dc.contributor.authorAbdulahad, W. H.
dc.contributor.authorSosicka, P.
dc.contributor.authorBijzet, J.
dc.contributor.authorLimburg, P. C.
dc.contributor.authorStegeman, C. A.
dc.contributor.authorBijl, M.
dc.contributor.authorWestra, J.
dc.contributor.authorKallenberg, C. G. M.
dc.contributor.institutionUniv Groningen
dc.contributor.institutionMartini Hosp
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Wroclaw
dc.date.accessioned2016-01-24T14:38:03Z
dc.date.available2016-01-24T14:38:03Z
dc.date.issued2014-11-01
dc.description.abstractThe objective of this study is to evaluate urinary high mobility group box 1 (HMGB1) levels as markers for active nephritis in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) in comparison with urinary CD4(+) effector memory T cells and urinary monocyte chemoattractant protein-1 (MCP-1). Twenty-four AAV patients with active nephritis and 12 healthy controls (HC) were evaluated. in nine patients, samples were also obtained during remission. Urinary levels of HMGB1 were measured by Western blot. CD4(+) T cells and CD4(+) effector memory T cells (CD4(+)CD45RO(+)CCR7(-)) were determined in urine and whole blood by flow cytometry. Measurement of urinary levels of MCP-1 and serum HMGB1 levels were performed by enzyme-linked immunosorbent assay (ELISA). AAV patients with active nephritis had higher median intensity of HMGB1 in urine than HC [10.3 (7.05-18.50) versus 5.8 (4.48-7.01); P = 0.004]. Both urinary HMGB1 and MCP-1 levels decreased significantly from active nephritis to remission. the urinary MCP-1/creatinine ratio correlated with Birmingham Vasculitis Activity Score (BVAS) (P = 0.042). No correlation was found between the HMGB1/creatinine ratio and 24-h proteinuria, estimated glomerular filtration rate (eGFR), MCP-1/creatinine ratio, BVAS and serum HMGB1. A positive correlation was found between urinary HMGB1/creatinine ratio and CD4(+) T cells/creatinine ratio (P = 0.028) and effector memory T cells/creatinine ratio (P = 0.039) in urine. Urinary HMGB1 levels are increased in AAV patients with active nephritis when compared with HC and patients in remission, and urinary HMGB1 levels are associated with CD4(+) T cells and CD4(+) effector memory T cells in urine. Measurement of urinary HMGB1 may be of additional value in identifying active glomerulonephritis in AAV patients.en
dc.description.affiliationUniv Groningen, Univ Med Ctr Groningen, Dept Rheumatol & Clin Immunol, NL-9713 GZ Groningen, Netherlands
dc.description.affiliationUniv Groningen, Univ Med Ctr Groningen, Dept Lab Med, NL-9713 GZ Groningen, Netherlands
dc.description.affiliationUniv Groningen, Univ Med Ctr Groningen, Dept Internal Med, Div Nephrol, NL-9713 GZ Groningen, Netherlands
dc.description.affiliationMartini Hosp, Dept Internal Med & Rheumatol, Groningen, Netherlands
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Internal Med, Div Rheumatol, São Paulo, Brazil
dc.description.affiliationUniv Wroclaw, Fac Biotechnol, Biochem Lab, PL-50138 Wroclaw, Poland
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Internal Med, Div Rheumatol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipResearch Institute for Drug Exploration (GUIDE)
dc.format.extent270-278
dc.identifierhttp://dx.doi.org/10.1111/cei.12422
dc.identifier.citationClinical and Experimental Immunology. Hoboken: Wiley-Blackwell, v. 178, n. 2, p. 270-278, 2014.
dc.identifier.doi10.1111/cei.12422
dc.identifier.issn0009-9104
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38364
dc.identifier.wosWOS:000342915800009
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofClinical and Experimental Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectanti-neutrophil cytoplasmic antibody-associated vasculitisen
dc.subjectglomerulonephritisen
dc.subjectHMGB1en
dc.subjectmonocyte chemoattractant protein-1en
dc.subjectT cellsen
dc.titleAre urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis?en
dc.typeinfo:eu-repo/semantics/article
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