High-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegypti

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dc.contributor.authorTorquato, Ricardo J. S. [UNIFESP]
dc.contributor.authorLu, Stephen [UNIFESP]
dc.contributor.authorMartins, Nadia Helena
dc.contributor.authorTanaka, Aparecida S. [UNIFESP]
dc.contributor.authorBarbosa Pereira, Pedro Jose
dc.date.accessioned2019-08-19T11:50:06Z
dc.date.available2019-08-19T11:50:06Z
dc.date.issued2017
dc.description.abstractBlood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single-domain, noncanonical Kazal-type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 angstrom resolution crystal structure of AaTI is reported from extremely tightly packed crystals (similar to 22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule.en
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Escola Paulista Med, Dept Biochem, Rua 3 Maio 100, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationLab Nacl Biociencias LNBio, Caixa Postal 6192, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Porto, IBMC, Rua Alfredo Allen 208, P-4200135 Oporto, Portugal
dc.description.affiliationUniv Porto, Inst Invest & Inovacao Saude, Rua Alfredo Allen 208, P-4200135 Oporto, Portugal
dc.description.affiliationUnifespUniv Fed São Paulo, UNIFESP, Escola Paulista Med, Dept Biochem, Rua 3 Maio 100, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipSpecial Visiting Researcher Program (PVE - Bolsa Pesquisador Visitante Especial)
dc.description.sponsorshipFCT - Fundação para a Ciencia e a Tecnologia in the form of the 'Institute for Research and Innovation in Health Sciences' project
dc.description.sponsorshipEuropean Regional Development Fund (FEDER) through the COMPETE - Operational Programme for Competitiveness and Internationalization (POCI), PORTUGAL
dc.description.sponsorshipStructured program on bioengineered therapies for infectious diseases and tissue regeneration
dc.description.sponsorshipNorte Portugal Regional Operational Programme
dc.description.sponsorshipFEDER
dc.description.sponsorshipCNPq
dc.description.sponsorshipIDFAPESP: 12/03657-8
dc.description.sponsorshipIDCNPq: 478504/2013-6
dc.description.sponsorshipIDCNPq: 400835/2014-2
dc.description.sponsorshipIDCNPq: 308780/2013-2
dc.description.sponsorshipIDPVE: 400835/2014-2
dc.description.sponsorshipIDFCT: POCI-01-0145-FEDER-007274
dc.description.sponsorshipIDStructured program on bioengineered therapies for infectious diseases and tissue regeneration: Norte-01-0145-FEDER-000012
dc.description.sponsorshipIDNORTE: 2020
dc.format.extent469-475
dc.identifierhttp://dx.doi.org/10.1107/S2053230X17010007
dc.identifier.citationActa Crystallographica Section F-Structural Biology Communications. Chester, v. 73, p. 469-475, 2017.
dc.identifier.doi10.1107/S2053230X17010007
dc.identifier.issn2053-230X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51475
dc.identifier.wosWOS:000406871600005
dc.language.isoeng
dc.publisherInt Union Crystallography
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectanticoagulantsen
dc.subjectthrombinen
dc.subjecttrypsinen
dc.subjectsalivary glandsen
dc.subjectprotein-protein interactionsen
dc.subjectKazal inhibitoren
dc.subjectAedes aegyptien
dc.subjectDengue feveren
dc.subjectYellow feveren
dc.subjectZika virusen
dc.titleHigh-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegyptien
dc.typeinfo:eu-repo/semantics/article
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