Growth-associated phosphoprotein expression is increased in the supragranular regions of the dentate gyrus following pilocarpine-induced seizures in rats

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dc.contributor.authorNaffah-Mazzacoratti, M. G.
dc.contributor.authorFunke, M. G.
dc.contributor.authorSanabria, ERG
dc.contributor.authorCavalheiro, E. A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:44Z
dc.date.available2016-01-24T12:30:44Z
dc.date.issued1999-01-01
dc.description.abstractNeuroplasticity has been investigated considering the neuronal growth-associated phosphoprotein as a marker of neuronal adaptive capabilities. in the present work, studying the hippocampal reorganization observed in the epilepsy model induced by pilocarpine, we carried out quantitative western blotting associated with immunohistochemistry to determine the distribution of growth-associated phosphoprotein in the hippocampus of rats in acute, silent and chronic periods of this epilepsy model. the fibers and punctate elements from the inner molecular layer of the dentate gyrus were strongly immunostained in animals killed 5 h after status epilepticus, compared with the same region in control animals. Rats presenting partial seizures showed no alterations in the immunostaining pattern compared with saline-treated animals. the hippocampal dentate gyrus of animals during the seizure-free period and presenting spontaneous recurrent seizures was also characterized by strong growth-associated phosphoprotein immunostaining of fibers and punctate elements in the inner molecular layer, contrasting with the control group. As determined by western blotting analysis, growth-associated phosphoprotein levels increased following status epilepticus and remained elevated at the later time-points, both during the silent period and during the period of chronic recurring seizures. Pilocarpine-treated animals, which did not develop status epilepticus, showed no change in growth-associated phosphoprotein levels, indicating that status epilepticus is important to induce growth-associated phosphoprotein overexpression.The measurement of this overexpression could represent one of the early signals of hippocampal reorganization due to status epilepticus-induced damage. (C) 1999 IBRO. Published by Elsevier B.V.en
dc.description.affiliationEscola Paulista Med, UNIFESP, Disciplina Neurol Expt, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationEscola Paulista Med, UNIFESP, Disciplina Bioquim, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationUnifespEscola Paulista Med, UNIFESP, Disciplina Neurol Expt, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationUnifespEscola Paulista Med, UNIFESP, Disciplina Bioquim, BR-04023900 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent485-492
dc.identifierhttp://dx.doi.org/10.1016/S0306-4522(98)00631-9
dc.identifier.citationNeuroscience. Oxford: Pergamon-Elsevier B.V., v. 91, n. 2, p. 485-492, 1999.
dc.identifier.doi10.1016/S0306-4522(98)00631-9
dc.identifier.issn0306-4522
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26008
dc.identifier.wosWOS:000079977200008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNeuroscience
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectGAP-43en
dc.subjecthippocampusen
dc.subjectmossy fiber sproutingen
dc.subjectpilocarpineen
dc.subjectepilepsyen
dc.titleGrowth-associated phosphoprotein expression is increased in the supragranular regions of the dentate gyrus following pilocarpine-induced seizures in ratsen
dc.typeinfo:eu-repo/semantics/article
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