BmSI-7, a novel subtilisin inhibitor from Boophilus microplus, with activity toward Pr1 proteases from the fungus Metarhizium anisopliae

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dc.contributor.authorSasaki, Sergio D. [UNIFESP]
dc.contributor.authorLima, Cdssia A. de [UNIFESP]
dc.contributor.authorLovato, Diogo V. [UNIFESP]
dc.contributor.authorJuliano, Maria A. [UNIFESP]
dc.contributor.authorTorquato, Ricardo J. S. [UNIFESP]
dc.contributor.authorTanaka, Aparecida S. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:32Z
dc.date.available2016-01-24T13:49:32Z
dc.date.issued2008-02-01
dc.description.abstractBmSI-7 and BmSI-6, two Boophilus microplus subtilisin inhibitors (BmSI) were purified and characterized from eggs. the inhibitors isolated by classical purification methods presented molecular masses of 7408 and 7271 Da, respectively, by MALDI-TOF-MS. Both BmSI-7 and BmSI-6 inhibited neutrophil elastase (K-i 0.4 and 0.3 nM) and subtilisin A (K-i 1.4 nM for both inhibitors). They also strongly inhibited Pr1 proteases from the fungus Metarhizium anisopliae; BmSI-7 (K-i 50 nM) and BmSI-6 (K-i 2.2 nM). the BmSI-7 full length cDNA was obtained using amino acid sequence information of BmSI-7 peptides generated by proteolytic digestion. BmSI-7 belongs to trypsin inhibitor like cysteine rich domain family (TIL), and it is transcribed in ovary, fat body, gut, salivary gland and haemocytes. BmSI-7 is the first TIL inhibitor described with inhibitory activity toward subtilisin A and Pr1 proteases of entomopathogenic fungi. (C) 2007 Elsevier Inc. All rights reserved.en
dc.description.affiliationUNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUNIFESP EPM, Dept Biofis, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Biofis, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent214-220
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2007.08.003
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 118, n. 2, p. 214-220, 2008.
dc.identifier.doi10.1016/j.exppara.2007.08.003
dc.identifier.issn0014-4894
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30414
dc.identifier.wosWOS:000252982400010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBoophilus microplusen
dc.subjectprotease inhibitoren
dc.subjectserine proteaseen
dc.subjectegg proteinsen
dc.subjectticken
dc.subjectMetarhizium anisopliae proteaseen
dc.subjectfungien
dc.titleBmSI-7, a novel subtilisin inhibitor from Boophilus microplus, with activity toward Pr1 proteases from the fungus Metarhizium anisopliaeen
dc.typeinfo:eu-repo/semantics/article
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