Conformational Properties of Angiotensin II and Its Active and Inactive TOAC-Labeled Analogs in the Presence of Micelles. Electron Paramagnetic Resonance, Fluorescence, and Circular Dichroism Studies

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dc.contributor.authorVieira, Renata de Freitas Fischer [UNIFESP]
dc.contributor.authorCasallanovo, Fabio
dc.contributor.authorMarin, Nelida
dc.contributor.authorPaiva, Antonio Cechelli de Mattos [UNIFESP]
dc.contributor.authorSchreier, Shirley
dc.contributor.authorNakaie, Clovis Ryuichi [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:02Z
dc.date.available2016-01-24T13:52:02Z
dc.date.issued2009-01-01
dc.description.abstractThe interaction between angiotensin II (AII, DRVYIHPF) and its analogs carrying 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) and detergents-negatively charged sodium dodecyl sulfate (SDS) and zwitterionic N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (HPS)-was examined by means of EPR, CD, and fluorescence. EPR spectra of partially active TOAC(1)-AII and inactive TOAC(3)-AII in aqueous solution indicated fast tumbling, the freedom of motion being greater at the N-terminus. Line broadening occurred upon interaction with micelles. Below SDS critical micelle concentration, broader lines indicated complex formation with tighter molecular packing than in micelles. Small changes in hyperfine splittings evinced TOAC location at the micelle-water interface. the interaction with anionic micelles was more effective than with zwitterionic micelles. Peptide-micelle interaction caused fluorescence increase. the TOAC-promoted intramolecular fluorescence quenching was more, pronounced for TOAC(3)-AII because of the proximity between the nitroxide and Tyr(4). CD spectra showed that although both AII and TOAC(1)-AII presented flexible conformations in water, TOAC(3)-AII displayed conformational restriction because of the TOAC-imposed bend (Schreier et al., Biopolymers 2004, 74, 389). in HPS, conformational changes were observed for the labeled peptides at neutral and basic pH. in SDS, all peptides underwent pH-dependent conformational changes. Although the spectra suggested similar folds for All and TOAC(1)-AII, different conformations were acquired by TOAC(3)-AII. the membrane environment has been hypothesized to shift conformational equilibria so as to stabilize the receptor-bound conformation of ligands. the fact that TOAC(3)-AII is unable to acquire conformations similar to those of native AII and partially active TOAC(1)-AII is probably the explanation for its lack of biological activity. (C) 2009 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 92: 525-537, 2009.en
dc.description.affiliationUniv São Paulo, Struct Biol Lab, Dept Biochem, Inst Chem, BR-05513970 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent525-537
dc.identifierhttps://dx.doi.org/10.1002/bip.21295
dc.identifier.citationBiopolymers. Hoboken: John Wiley & Sons Inc, v. 92, n. 6, p. 525-537, 2009.
dc.identifier.doi10.1002/bip.21295
dc.identifier.issn0006-3525
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31142
dc.identifier.wosWOS:000272364900007
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBiopolymers
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectAngiotensin IIen
dc.subjectCDen
dc.subjectEPRen
dc.subjectMicelleen
dc.subjectPeptide conformationen
dc.subjectSpin labelen
dc.subjectTOACen
dc.subjectStructure-activity relationshipen
dc.titleConformational Properties of Angiotensin II and Its Active and Inactive TOAC-Labeled Analogs in the Presence of Micelles. Electron Paramagnetic Resonance, Fluorescence, and Circular Dichroism Studiesen
dc.typeinfo:eu-repo/semantics/article
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