Pro-epileptic effect of alfentanil in rats subjected to pilocarpine-induced chronic epilepsy

Página do item simplificado
dc.contributor.authorGarrido Sanabria, Emilio Rafael
dc.contributor.authorD'Andrea Vieira, Isabella
dc.contributor.authorFernanda Da Silveira Pereira, Maria
dc.contributor.authorCoutinho Faria, Leonardo
dc.contributor.authorDa Silva, Andre Cesar
dc.contributor.authorAbrao Cavalheiro, Esper
dc.contributor.authorSilva Fernandes, Maria Jose da
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Brownsville
dc.date.accessioned2016-01-24T12:41:11Z
dc.date.available2016-01-24T12:41:11Z
dc.date.issued2006-05-15
dc.description.abstractPharmacological induction of epileptiform activity is a complementary method to study the epileptogenic area in drug-resistant epileptic patients. Among the different activation methods, fentanyl derivatives (e.g. alfentanil) provide one of the most efficient tools in triggering epileptiform abnormalities in surgical candidates. in this study, we tested the pro-epileptic effect of different concentrations of alfentanil in hippocampal slices obtained from control and pilocarpine-treated chronic epileptic rats. the pro-convulsant action of alfentanil was also studied in control and pilocarpine-treated epileptic rats implanted with subdural and hippocampal electrodes for electroencephalographic recordings. in 90% of slices from control animals, application of affentanil (0.1-5 mu M) induced a significant enhancement in amplitude and number of population spikes recorded in the hippocampal CA1 region. in contrast, alfentanil produced a significant reduction in the amplitude of population spikes in slices from pilocarpine-treated epileptic rats. These changes were accompanied by a significant increase in the number of population spikes in the form of epileptiform multispike responses of epileptic slices. Naloxone (20 mu M) antagonized the effect of alfentanil in both control and epileptic slices, reducing the number of population spikes in slices from epileptic rats. in control rats, alfentanil induced epileptiform abnormalities in the hippocampal and cortical electroencephalographic recordings but only at concentrations higher than 200 [mu g/kg (e.g. 350 mu g/kg). Lower doses of affentanil (25 mu g/kg) elicited epileptiform abnormalities only in chronic epileptic rats. the potent action of a minimal dose of alfentanil in inducing epileptiform activity suggests an enhancement of the pro-convulsant action of mu-receptor opioids in chronic temporal lobe epilepsy. (c) 2006 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Neurol & Neurocirurgia, São Paulo, Brazil
dc.description.affiliationUniv Brownsville, Dept Sci Biol, Brownsville, TX USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Neurol & Neurocirurgia, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent535-545
dc.identifierhttp://dx.doi.org/10.1016/j.brainresbull.2006.02.016
dc.identifier.citationBrain Research Bulletin. Oxford: Pergamon-Elsevier B.V., v. 69, n. 5, p. 535-545, 2006.
dc.identifier.doi10.1016/j.brainresbull.2006.02.016
dc.identifier.issn0361-9230
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28909
dc.identifier.wosWOS:000237678000007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research Bulletin
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectopioiden
dc.subjectfentanylen
dc.subjectseizuresen
dc.subjecthippocampusen
dc.subjectnaloxoneen
dc.subjecttemporal lobe epilepsyen
dc.titlePro-epileptic effect of alfentanil in rats subjected to pilocarpine-induced chronic epilepsyen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos