FURTHER CHARACTERIZATION OF THE ACIDIC GPI-HYDROLYZING PHOSPHOLIPASE PRESENT IN HUMAN SERA
| dc.contributor.author | Stambuk, Boris Juan Carlos Ugarte [UNIFESP] | |
| dc.contributor.author | Cardosodealmeida, M. L. | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2018-06-15T14:28:06Z | |
| dc.date.available | 2018-06-15T14:28:06Z | |
| dc.date.issued | 1994-02-01 | |
| dc.description.abstract | A phospholipase from human serum capable of hydrolyzing glycosylphosphatidylinositol membrane anchors was described and partially characterized by our group some years ago. This activity presented a pH optimum between 5.0 and 6.0 and was inhibited by EDTA, EGTA and 1,10-phenanthroline. Partial purification showed that the enzyme was a glycoprotein with an apparent molecular weight of 140 kDa as judged by gel filtration. Other investigators characterized at the same time a phospholipase D with similar properties but with a pH optimum near 7.5. We now confirm that the human serum enzyme is indeed a phospholipase D capable of hydrolyzing mfVSG and glycolipids A and C from T. brucei. Isoelectric focusing of whole sera suggests the presence of two isoforms, one with a pi of 4.7 which was the form previously purified by our group, and others with pI from 6.2 to 7.4. | en |
| dc.description.affiliation | ESCOLA PAULISTA MED,DEPT MICROBIOL IMUNOL & PARASITOL,BR-04023062 SAO PAULO,SP,BRAZIL | |
| dc.description.affiliationUnifesp | ESCOLA PAULISTA MED,DEPT MICROBIOL IMUNOL & PARASITOL,BR-04023062 SAO PAULO,SP,BRAZIL | |
| dc.description.source | Web of Science | |
| dc.format.extent | 383-387 | |
| dc.identifier.citation | Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 2, p. 383-387, 1994. | |
| dc.identifier.issn | 0100-879X | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/43052 | |
| dc.identifier.wos | WOS:A1994MX60900040 | |
| dc.language.iso | eng | |
| dc.publisher | Assoc Bras Divulg Cientifica | |
| dc.relation.ispartof | Brazilian Journal Of Medical And Biological Research | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.subject | GPI | en |
| dc.subject | PHOSPHOLIPASE D | en |
| dc.subject | ACIDIC LIPASE | en |
| dc.title | FURTHER CHARACTERIZATION OF THE ACIDIC GPI-HYDROLYZING PHOSPHOLIPASE PRESENT IN HUMAN SERA | en |
| dc.type | info:eu-repo/semantics/article |