Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo

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dc.contributor.authorVenters, Sara J.
dc.contributor.authorDias-da-Silva, Magnus Régios [UNIFESP]
dc.contributor.authorHyer, Jeanette
dc.contributor.institutionUniv Calif San Francisco
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:51:47Z
dc.date.available2016-01-24T13:51:47Z
dc.date.issued2008-11-01
dc.description.abstractWe describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; SNV) promoter or the chick beta-actin promoter, into the backbone of the murine leukemia (MLV) viral vector. the additional promoters drive transgene expression in avian tissue. the remainder of the vector is MLV-like, allowing high titer viral particle production by means of transient transfection. the SNV promoter produces high and early expression of introduced genes, enabling detection of the single copy integrated GFP gene in infected cells and their progeny in vivo. Substitution of the LacZ coding DNA with a relevant gene of interest will enable its co-expression with GFP, thus allowing visualization of the effect of specific and stable changes in gene expression throughout development. As the VSV-G pseudotyped viral vector is replication incompetent, changes in gene expression can be controlled temporally, by altering the timing of introduction. Developmental Dynamics 237.3260-3269, 2008. Published 2008 Wiley-Liss, Inc.en
dc.description.affiliationUniv Calif San Francisco, Dept Neurosurg, San Francisco, CA 94143 USA
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent3260-3269
dc.identifierhttp://dx.doi.org/10.1002/dvdy.21766
dc.identifier.citationDevelopmental Dynamics. Hoboken: Wiley-liss, v. 237, n. 11, p. 3260-3269, 2008.
dc.identifier.doi10.1002/dvdy.21766
dc.identifier.issn1058-8388
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30974
dc.identifier.wosWOS:000260930400016
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofDevelopmental Dynamics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectretrovirusen
dc.subjectSNVen
dc.subjectchicken embryoen
dc.subjectgene expressionen
dc.subjectlineage tracingen
dc.subjectembryonic developmenten
dc.titleMurine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryoen
dc.typeinfo:eu-repo/semantics/article
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