Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor

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dc.contributor.authorCarvalho, Lucimeire Nova de [UNIFESP]
dc.contributor.authorCristovam, Priscila Cardoso [UNIFESP]
dc.contributor.authorPassos, Clévia dos Santos [UNIFESP]
dc.contributor.authorBoim, Mirian Aparecida [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:38Z
dc.date.available2016-01-24T14:17:38Z
dc.date.issued2012-01-01
dc.description.abstractBackground/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, Baselen
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao Oswaldo Ramos (FOR)
dc.format.extent1456-1464
dc.identifierhttp://dx.doi.org/10.1159/000343333
dc.identifier.citationCellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012.
dc.identifier.doi10.1159/000343333
dc.identifier.fileWOS000314149200012.pdf
dc.identifier.issn1015-8987
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34389
dc.identifier.wosWOS:000314149200012
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCellular Physiology and Biochemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectPregnancyen
dc.subjectMesangial cellsen
dc.subjectIntracellular calciumen
dc.subjectRelaxinen
dc.subjectAT2 receptoren
dc.subjectNitric oxideen
dc.titleMesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptoren
dc.typeinfo:eu-repo/semantics/article
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