Sleep is not just for the brain: transcriptional responses to sleep in peripheral tissues

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dc.contributor.authorAnafi, Ron C.
dc.contributor.authorPellegrino, Renata [UNIFESP]
dc.contributor.authorShockley, Keith R.
dc.contributor.authorRomer, Micah
dc.contributor.authorTufik, Sergio [UNIFESP]
dc.contributor.authorPack, Allan I.
dc.contributor.institutionUniv Penn
dc.contributor.institutionChildrens Hosp Philadelphia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionNIEHS
dc.date.accessioned2016-01-24T14:31:47Z
dc.date.available2016-01-24T14:31:47Z
dc.date.issued2013-05-30
dc.description.abstractBackground: Many have assumed that the primary function of sleep is for the brain. We evaluated the molecular consequences of sleep and sleep deprivation outside the brain, in heart and lung. Using microarrays we compared gene expression in tissue from sleeping and sleep deprived mice euthanized at the same diurnal times.Results: in each tissue, nearly two thousand genes demonstrated statistically significant differential expression as a function of sleep/wake behavioral state. To mitigate the influence of an artificial deprivation protocol, we identified a subset of these transcripts as specifically sleep-enhanced or sleep-repressed by requiring that their expression also change over the course of unperturbed sleep. 3% and 6% of the assayed transcripts showed sleep specific changes in the lung and heart respectively. Sleep specific transcripts in these tissues demonstrated highly significant overlap and shared temporal dynamics. Markers of cellular stress and the unfolded protein response were reduced during sleep in both tissues. These results mirror previous findings in brain. Sleep-enhanced pathways reflected the unique metabolic functions of each tissue. Transcripts related to carbohydrate and sulfur metabolic processes were enhanced by sleep in the lung, and collectively favor buffering from oxidative stress. DNA repair and protein metabolism annotations were significantly enriched among the sleep-enhanced transcripts in the heart. Our results also suggest that sleep may provide a Zeitgeber, or synchronizing cue, in the lung as a large cluster of transcripts demonstrated systematic changes in inter-animal variability as a function of both sleep duration and circadian time.Conclusion: Our data support the notion that the molecular consequences of sleep/wake behavioral state extend beyond the brain to include peripheral tissues. Sleep state induces a highly overlapping response in both heart and lung. We conclude that sleep enhances organ specific molecular functions and that it has a ubiquitous role in reducing cellular metabolic stress in both brain and peripheral tissues. Finally, our data suggest a novel role for sleep in synchronizing transcription in peripheral tissues.en
dc.description.affiliationUniv Penn, Perelman Sch Med, Div Sleep Med, Philadelphia, PA 19104 USA
dc.description.affiliationUniv Penn, Perelman Sch Med, Ctr Sleep & Circadian Neurobiol, Philadelphia, PA 19104 USA
dc.description.affiliationChildrens Hosp Philadelphia, Ctr Appl Genom, Philadelphia, PA 19104 USA
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, São Paulo, Brazil
dc.description.affiliationNIEHS, US Dept HHS, Biostat Branch, NIH, Res Triangle Pk, NC 27709 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, EPM, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipIntramural Research Program of the National Institutes of Health, National Institute of Environmental Health Sciences
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipAmerican Sleep Medicine Foundation
dc.description.sponsorshipNational Heart Lung Blood Institute
dc.description.sponsorshipIDNational Institutes of Health: AG-17628
dc.description.sponsorshipIDNational Heart Lung Blood Institute: HL090021
dc.description.sponsorshipID: K12
dc.format.extent19
dc.identifierhttp://dx.doi.org/10.1186/1471-2164-14-362
dc.identifier.citationBmc Genomics. London: Biomed Central Ltd, v. 14, 19 p., 2013.
dc.identifier.doi10.1186/1471-2164-14-362
dc.identifier.fileWOS000321386100002.pdf
dc.identifier.issn1471-2164
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36352
dc.identifier.wosWOS:000321386100002
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Genomics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSleepen
dc.subjectCircadian Rhythmsen
dc.subjectSleep Deprivationen
dc.subjectUnfolded Protein Responseen
dc.subjectHearten
dc.subjectLungen
dc.subjectSynchronizationen
dc.titleSleep is not just for the brain: transcriptional responses to sleep in peripheral tissuesen
dc.typeinfo:eu-repo/semantics/article
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