Keloid explant culture: a model for keloid fibroblasts isolation and cultivation based on the biological differences of its specific regions

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dc.contributor.authorTucci-Viegas, Vanina Monique [UNIFESP]
dc.contributor.authorHochman, Bernardo [UNIFESP]
dc.contributor.authorFranca, Jeronimo P. [UNIFESP]
dc.contributor.authorFerreira, Lydia M. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:33Z
dc.date.available2016-01-24T14:05:33Z
dc.date.issued2010-10-01
dc.description.abstractIn vitro studies with keloid fibroblasts frequently present contradictory results. This may occur because keloids present distinct genotypic and phenotypic characteristics in its different regions, such as the peripheral region in relation to the central region. We suggest an explant model for keloid fibroblasts harvesting, standardising the initial processing of keloid samples to obtain fragments from different regions, considering its biological differences, for primary cell culture. the different keloid regions were delimited and fragments were obtained using a 3-mm diameter punch. To remove fragments from the periphery, the punch was placed in one longitudinal line extremity, respecting the lesion borders. for the central region, it was placed in the intersection of lines at the level of the largest longitudinal and transversal axes, the other fragments being removed centrifugally in relation to the first one. Primary fibroblast culture was carried out by explant. Flow cytometry analysis showed cell cycle differences between the groups, confirming its different origins and biological characteristics. in conclusion, our proposed model proved itself efficient for keloid fibroblast isolation from specific regions and cultivation. Its simplicity and ease of execution may turn it into an important tool for studying the characteristics of the different keloid-derived fibroblasts in culture.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Plast Surg, Lab Cell Culture, BR-04024900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol, BR-04024900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Plast Surg, Lab Cell Culture, BR-04024900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol, BR-04024900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent339-348
dc.identifierhttp://dx.doi.org/10.1111/j.1742-481X.2010.00698.x
dc.identifier.citationInternational Wound Journal. Malden: Wiley-Blackwell Publishing, Inc, v. 7, n. 5, p. 339-348, 2010.
dc.identifier.doi10.1111/j.1742-481X.2010.00698.x
dc.identifier.issn1742-4801
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32980
dc.identifier.wosWOS:000287514300005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Wound Journal
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectCell culture techniquesen
dc.subjectcultured cellsen
dc.subjectfibroblastsen
dc.subjectkeloiden
dc.subjectin vitroen
dc.titleKeloid explant culture: a model for keloid fibroblasts isolation and cultivation based on the biological differences of its specific regionsen
dc.typeinfo:eu-repo/semantics/article
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