Ionic and biochemical characterization of bovine intervertebral disk

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dc.contributor.authorMartins, Delio Eulalio [UNIFESP]
dc.contributor.authorMedeiros, Valquiria Pereira
dc.contributor.authorDemerov, Gabriela Feitosa [UNIFESP]
dc.contributor.authorAccardo, Camila Melo [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar Julian [UNIFESP]
dc.contributor.authorWajchenberg, Marcelo [UNIFESP]
dc.contributor.authorReginato, Rejane Daniele [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorPuertas, Eduardo Barros [UNIFESP]
dc.contributor.authorFaloppa, Flavio [UNIFESP]
dc.date.accessioned2019-01-21T10:29:59Z
dc.date.available2019-01-21T10:29:59Z
dc.date.issued2016
dc.description.abstractIntroduction: Intervertebral disks have been associated with low back pain, and many therapies have been proposed for its treatment. The cellular and molecular knowledge of intervertebral disks composition and precise methods to quantify disk components are important for any type of proposed therapy. Thus, the aim of this study was to correlate glycosaminoglycans presence with the quantitation of cells, ions and collagen fiber distributions in different intervertebral disk sections. Methods: In total, 14 intervertebral disks were used from cattle. All of the disks were dehydrated, separated in seven sections and digested in sodium-free papain buffer. Glycosaminoglycan measurements were performed in the samples according to agarose electrophoresis methoden
dc.description.abstracttotal cells were measured using the PicoGreen (R) technique, ions were quantified, and collagen fiber birefringence was analyzed with polarized light. Results: Cations Na+ and K+ are more concentrate in the nucleus (Na+ = 1688.50 +/- 110 mmol/Len
dc.description.abstractK+ = 111.9 +/- 28 mmol/L) of intervertebral disks than the annulus (Na+ = 652.80 +/- 75 mmol/Len
dc.description.abstractK+ = 55.6 +/- 8 mmol/L). A negative correlation between cells number and sodium/potassium was observed (p < 0.001) Additionally, thin collagen fibers were largest in the nucleus, similar to hyaluronate distribution. Conclusions: The results suggest that annulus fibrosus cells are also sensitive to changes in ionic concentrations such as nucleus pulposus cells. Additionally, hyaluronate is related to thin collagen fibers type II.en
dc.description.affiliationDepartment of Orthopaedics and Traumatology, Escola Paulista de Medicina , Universidade Federal de São Paulo , São Paulo , Brazil
dc.description.affiliationDepartment of Biochemistry , Universidade Federal de Juiz de Fora , Minas Gerais , Brazil
dc.description.affiliationDepartment of Biochemistry, Escola Paulista de Medicina , Universidade Federal de São Paulo , São Paulo , Brazil
dc.description.affiliationCentro Interdisciplinar de Investigacao Bioquimica , Universidade de Mogi das Cruzes , Mogi das Cruzes , Brazil
dc.description.affiliationDepartment of Morphology and Genetics, Escola Paulista de Medicina , Universidade Federal de São Paulo , São Paulo , Brazil
dc.description.affiliationUnifespDepartment of Orthopaedics and Traumatology, Escola Paulista de Medicina , Universidade Federal de São Paulo , São Paulo , Brazil
dc.description.affiliationUnifespDepartment of Biochemistry, Escola Paulista de Medicina , Universidade Federal de São Paulo , São Paulo , Brazil
dc.description.affiliationUnifespDepartment of Morphology and Genetics, Escola Paulista de Medicina , Universidade Federal de São Paulo , São Paulo , Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2009/54793-6]
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
dc.description.sponsorshipIDFAPESP: 2009/54793-6
dc.format.extent212-219
dc.identifierhttps://doi.org/10.3109/03008207.2016.1140751
dc.identifier.citationConnective Tissue Research. Philadelphia, v. 57, n. 3, p. 212-219, 2016.
dc.identifier.doi10.3109/03008207.2016.1140751
dc.identifier.issn0300-8207
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49522
dc.identifier.wosWOS:000375235800005
dc.language.isoeng
dc.publisherTaylor & francis inc
dc.relation.ispartofConnective Tissue Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCollagenen
dc.subjectGlycosaminoglycansen
dc.subjectHyaluronic Aciden
dc.subjectIntervertebral Disken
dc.subjectPotassiumen
dc.subjectSodiumExtracellular-Matrixen
dc.subjectEnd-Plateen
dc.subjectAcidic Mucopolysaccharidesen
dc.subjectCartilageen
dc.subjectDnaen
dc.subjectHoechst-33258en
dc.subjectHyaluronanen
dc.subjectTherapyen
dc.subjectDensityen
dc.subjectAssayen
dc.titleIonic and biochemical characterization of bovine intervertebral disken
dc.typeinfo:eu-repo/semantics/article
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