Evaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with acai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assay

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dc.contributor.authorRibeiro, Juliana Carvalho
dc.contributor.authorGreggi Antunes, Lusania Maria
dc.contributor.authorAissa, Alexandre Ferro
dc.contributor.authorCastania Darin, Joana D'arc
dc.contributor.authorDe Rosso, Veridiana Vera [UNIFESP]
dc.contributor.authorMercadante, Adriana Zerlotti
dc.contributor.authorPires Bianchi, Maria de Lourdes
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2016-01-24T13:59:07Z
dc.date.available2016-01-24T13:59:07Z
dc.date.issued2010-01-01
dc.description.abstractAcai, the fruit of a palm native to the Amazonian basin, is widely distributed in northern South America, where it has considerable economic importance. Whereas individual polyphenolics compounds in Acai have been extensively evaluated, studies of the intact fruit and its biological properties are lacking. Therefore, the present study was undertaken to investigate the in vivo genotoxicity of Acai and its possible antigenotoxicity on doxorubicin (DXR)-induced DNA damage. the Acai pulp doses selected were 3.33, 10.0 and 16.67 g/kg b.w. administered by gavage alone or prior to DXR (16 mg/kg b.w.) administered by intraperitoneal injection. Swiss albino mice were distributed in eight groups for acute treatment with acai pulp (24 h) and eight groups for subacute treatment (daily for 14 consecutive days) before euthanasia. the negative control groups were treated in a similar way. the results of chemical analysis suggested the presence of carotenoids, anthocyanins, phenolic. and flavonoids in Acai pulp. the endpoints analyzed were micronucleus induction in bone marrow and peripheral blood cells polychromatic erythrocytes, and DNA damage in peripheral blood, liver and kidney cells assessed using the alkaline (pH > 13) comet assay. There were no statistically significant differences (p > 0.05) between the negative control and the groups treated with the three doses of Acai pulp alone in all endpoints analyzed, demonstrating the absence of genotoxic effects. the protective effects of Acai pulp were observed in both acute and subacute treatments, when administered prior to DXR. in general, subacute treatment provided greater efficiency in protecting against DXR-induced DNA damage in liver and kidney cells. These protective effects can be explained as the result of the phytochemicals present in Acai pulp. These results will be applied to the developmental of food with functional characteristics, as well as to explore the characteristics of Acai as a health promoter. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Saude, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Ciencia Alimentos, Fac Engn Alimentos, Campinas, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Saude, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent22-28
dc.identifierhttp://dx.doi.org/10.1016/j.mrgentox.2009.10.009
dc.identifier.citationMutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 695, n. 1-2, p. 22-28, 2010.
dc.identifier.doi10.1016/j.mrgentox.2009.10.009
dc.identifier.issn1383-5718
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32110
dc.identifier.wosWOS:000274874300005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMutation Research-genetic Toxicology and Environmental Mutagenesis
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectEuterpe oleraceaen
dc.subjectDoxorubicinen
dc.subjectAntigenotoxicen
dc.subjectMiceen
dc.subjectPolyphenolsen
dc.subjectAnthocyaninsen
dc.titleEvaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with acai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assayen
dc.typeinfo:eu-repo/semantics/article
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